Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb #4577

Flk2   FMS-related tyrosine kinase 3  

No. Size Price
4577S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
4577 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 160 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb detects endogenous levels of FLT3 only when phosphorylated at Tyr842. This antibody may cross-react with other tyrosine-phosphorylated family members.

磷酸化FLT3 (Tyr842) (10A8) 兔单抗仅在Tyr842被磷酸化后才能检测到内源FLT3的存在。本抗体可能与其它磷酸化的酪氨酸激酶发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence surrounding Tyr842 of human FLT3.


Western Blotting

Western Blotting

Western blot analysis of cell extracts from SEM cells expressing constitutively activated FLT3 using Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb (upper and middle). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. Total FLT3 (8F2) Rabbit mAb #3462 shown at the bottom. 检测组成型表达激活的FLT3的SEM细胞的提取物,使用的抗体为Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb(上,中)。中,抗体孵育前用intestinal phosphatase (CIP)处理;下,用抗体FLT3 (8F2) Rabbit mAb #3462检测的结果。


FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).

FMS-相关的酪氨酸激酶3 (FLT3, 也称Flk2)是III型酪氨酸激酶受体家族的成员之一,其余成员包括c-Kit, PDGFR 和M-CSF 受体。FLT3表达在早期的造血前体细胞上,并支持造血系统的发育和分化(1,2)。FLT3结合其配体FL后激活,进而导致了酪氨酸酶自磷酸化和下游靶点酪氨酸磷酸化的级联反应(3)。FL刺激之后,FLT3就和PI3K激酶的p85亚基、 SHP2、 GRB2和Shc相关联(4-6)。Tyr589/591定位于FLT3的近膜区并对FLT3的酪氨酸激酶活性的调控有重要作用。20%的急性骨髓性白血病病人中发生FLT3内部串联重复 (ITDs)体细胞突变(7)。

Tyr842 is located in the activation loop of FLT3. Mutation of Tyr842 was identified in human AML patients (8).

Tyr842 定位在FLT3的激活环区。急性髓细胞白血病(AML)病人 Tyr842位点被发现有突变 (8)。

  1. Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev 9, 37-48.
  2. Naoe, T. et al. (2001) Cancer Chemother Pharmacol 48 Suppl 1, S27-30.
  3. Namikawa, R. et al. (1996) Stem Cells 14, 388-95.
  4. Beslu, N. et al. (1996) J Biol Chem 271, 20075-81.
  5. Zhang, S. and Broxmeyer, H.E. (2000) Biochem Biophys Res Commun 277, 195-9.
  6. Zhang, S. et al. (1999) J Leukoc Biol 65, 372-80.
  7. Mizuki, M. et al. (2000) Blood 96, 3907-14.
  8. Kindler, T. et al. (2005) Blood 105, 335-340.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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