Cell Signaling Technology

Product Pathways - Protein Stability

Napsin A (D2G1Y) Rabbit mAb (IF Formulated) #43861

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IF-IC 1:100 Human, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Napsin A (D2G1Y) Rabbit mAb (IF Formulated) recognizes endogenous levels of total napsin A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human napsin A protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of H3255 (left) and 293T (right) cells using Napsin A (D2G1Y) Rabbit mAb (IF Formulated) (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Napsin A is an aspartic proteinase that is expressed in normal lung and kidney (1). In the lung, napsin A is expressed by type II pneumocytes and alveolar macrophages, where it plays a role in processing surfactant protein B (2). Napsin A is expressed in lung adenocarcinomas, where it can be used to identify primary and metastatic lesions with greater sensitivity compared to TTF-1 (3,4). Napsin A expression has also been described in other types of cancer, such as kidney and thyroid cancer (5).

  1. Tatnell, P.J. et al. (1998) FEBS Lett 441, 43-8.
  2. Schauer-Vukasinovic, V. et al. (1999) FEBS Lett 462, 135-9.
  3. Jagirdar, J. (2008) Arch Pathol Lab Med 132, 384-96.
  4. Turner, B.M. et al. (2012) Arch Pathol Lab Med 136, 163-71.
  5. Bishop, J.A. et al. (2010) Hum Pathol 41, 20-5.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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