Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

EPAC2 (D3P3J) Rabbit mAb #43239

No. Size Price
43239S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
43239 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 115 Rabbit IgG
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

EPAC2 (D3P3J) Rabbit mAb recognizes endogenous levels of total EPAC2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln975 of human EPAC2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissues uing EPAC2 (D3P3J) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower)

Background

EPAC1 and EPAC2 (exchange proteins activated by cyclic AMP) are guanine nucleotide exchange factors (GEFs) that catalyze the exchange of GDP for GTP, activating Rap1 and Rap2 small GTPases. Rap activation by EPAC is cAMP-dependent and mediates cAMP signaling in part through protein kinase A (PKA) (reviewed in 1). EPAC signaling plays a significant role in a number of cellular processes including migration and focal adhesion formation (2), exocytosis (3), insulin signaling (4), axon growth and guidance (5) and neurotransmitter release (6).

  1. Bos, J.L. (2006) Trends Biochem Sci 31, 680-6.
  2. Lyle, K.S. et al. (2008) Cell Signal 20, 1104-16.
  3. Branham, M.T. et al. (2009) J Biol Chem 284, 24825-39.
  4. Petersen, R.K. et al. (2008) Mol Cell Biol 28, 3804-16.
  5. Murray, A.J. and Shewan, D.A. (2008) Mol Cell Neurosci 38, 578-88.
  6. Ouyang, M. et al. (2008) Proc Natl Acad Sci USA 105, 11993-7.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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