Cell Signaling Technology

Product Pathways - Metabolism

NaPi2b/SLC34A2 (D6W2G) Rabbit mAb #42299

No. Size Price
42299S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
42299 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 73, 90-130 Rabbit IgG
IP 1:50
IHC-P 1:200
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

NaPi2b/SLC34A2 (D6W2G) Rabbit mAb

recognizes endogenous levels of total NaPi2b/SLC34A2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NaPi2b/SLC34A2 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of IGROV-1 (left) and Hep G2 (right) cells using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb (green). Actin filaments were labeled with DyLightTM 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from IGROV1, ACHN and HepG2 using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb and beta-Actin (13E5) Rabbit mAb #4970.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HCC78 (left) and Hep G2 (right) cell pellets using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb.

Background

The sodium-dependent phosphate transport protein 2B (NaPi-2b, SLC34A2) is a sodium dependent inorganic phosphate (Pi) transporter that regulates phosphate homeostasis in various organs, including the small intestine, lung, liver, and testis (1). In the small intestine, NaPi-2b localizes to the intestinal brush border membrane to mediate Pi reabsorption (2). In the lung, NaPi-2b is expressed in the apical membrane of type II alveolar cells and is involved in the synthesis of surfactant (3). Mutations in the corresponding SLC34A2 gene causes pulmonary alveolar microlithiasis, a rare autosomal recessive disorder characterized by the deposition of calcium phosphate microliths throughout the lungs (4). Research studies show aberrant expression of NaPi-2b in various type of cancer, including ovarian, breast, and lung cancer (5). Chromosomal rearrangements involving SLC34A2-ROS1 are seen in gastric carcinoma and non-small cell lung cancer and result in the formation of a SLC34A2-ROS1 chimeric protein that retains a constitutive kinase activity (6,7).

  1. Xu, H. et al. (1999) Genomics 62, 281-4.
  2. Hilfiker, H. et al. (1998) Proc Natl Acad Sci U S A 95, 14564-9.
  3. Traebert, M. et al. (1999) Am J Physiol 277, L868-73.
  4. Yin, X. et al. (2013) Respir Med 107, 217-22.
  5. Kiyamova, R. et al. (2011) Exp Oncol 33, 157-61.
  6. Davies, K.D. et al. (2012) Clin Cancer Res 18, 4570-9.
  7. Lee, J. et al. (2013) Cancer 119, 1627-35.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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