Product Pathways - Metabolism
NaPi2b/SLC34A2 (D6W2G) Rabbit mAb #42299
|42299S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|42299||carrier free & custom formulation / quantity||email request|
|W||1:1000||Human,||Endogenous||73, 90-130||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
NaPi2b/SLC34A2 (D6W2G) Rabbit mAbrecognizes endogenous levels of total NaPi2b/SLC34A2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human NaPi2b/SLC34A2 protein.
Confocal immunofluorescent analysis of IGROV-1 (left) and Hep G2 (right) cells using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb (green). Actin filaments were labeled with DyLightTM 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of extracts from IGROV1, ACHN and HepG2 using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb and beta-Actin (13E5) Rabbit mAb #4970.
Immunohistochemical analysis of paraffin-embedded HCC78 (left) and Hep G2 (right) cell pellets using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using NaPi2b/SLC34A2 (D6W2G) Rabbit mAb.
The sodium-dependent phosphate transport protein 2B (NaPi-2b, SLC34A2) is a sodium dependent inorganic phosphate (Pi) transporter that regulates phosphate homeostasis in various organs, including the small intestine, lung, liver, and testis (1). In the small intestine, NaPi-2b localizes to the intestinal brush border membrane to mediate Pi reabsorption (2). In the lung, NaPi-2b is expressed in the apical membrane of type II alveolar cells and is involved in the synthesis of surfactant (3). Mutations in the corresponding SLC34A2 gene causes pulmonary alveolar microlithiasis, a rare autosomal recessive disorder characterized by the deposition of calcium phosphate microliths throughout the lungs (4). Research studies show aberrant expression of NaPi-2b in various type of cancer, including ovarian, breast, and lung cancer (5). Chromosomal rearrangements involving SLC34A2-ROS1 are seen in gastric carcinoma and non-small cell lung cancer and result in the formation of a SLC34A2-ROS1 chimeric protein that retains a constitutive kinase activity (6,7).
- Xu, H. et al. (1999) Genomics 62, 281-4.
- Hilfiker, H. et al. (1998) Proc Natl Acad Sci U S A 95, 14564-9.
- Traebert, M. et al. (1999) Am J Physiol 277, L868-73.
- Yin, X. et al. (2013) Respir Med 107, 217-22.
- Kiyamova, R. et al. (2011) Exp Oncol 33, 157-61.
- Davies, K.D. et al. (2012) Clin Cancer Res 18, 4570-9.
- Lee, J. et al. (2013) Cancer 119, 1627-35.
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- 13079 Immunohistochemistry Application Solutions Kit (Rabbit)
- 14166 Hematoxylin
- 14177 SignalStain® Mounting Medium
- 14746 SignalStain® Citrate Unmasking Solution (10X)
- 15019 Animal-Free Blocking Solution (5X)
- 5425 Normal Goat Serum
- 66445 NaPi2b/SLC34A2 (D3V3I) Rabbit mAb
- 8059 SignalStain® DAB Substrate Kit
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
For Research Use Only. Not For Use In Diagnostic Procedures.
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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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SignalStain is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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