Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-Cyclin E1 (Thr62) Antibody #4136

No. Size Price
4136S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
4136 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 48 Rabbit
IP 1:100
IHC-P 1:100
F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

Phospho-Cyclin E (Thr62) Antibody detects endogenous levels of cyclin E only when phosphorylated at threonine 62 (cyclin E1 isoform 2) or threonine 77 (cyclin E1 isoform 1). Phospho-Cyclin E (Thr62) Antibody能够检测内源性的苏氨酸62位点(cyclin E1 isoform 2)或苏氨酸77位点(cyclin E1 isoform 1)磷酸化的cyclin E蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr62 of human cyclin E. Antibodies are purified by protein protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对cyclin E蛋白苏氨酸(62位)磷酸化肽段免疫动物,利用A蛋白和多肽亲和层析方法纯化生产的。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-Cyclin E1 (Thr62) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-Cyclin E (Thr62)-positive cells.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing nuclear localization, using Phospho-Cyclin E (Thr62) Antibody. 免疫组织化学方法检测石蜡包埋人类结肠癌组织,显示核定位,检测抗体为Phospho-Cyclin E (Thr62) Antibody。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-Cyclin E (Thr62) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Cyclin E (Thr62) Antibody in the presence of control peptide (left) or antigen-specific peptide (right). 免疫组织化学方法检测石蜡包埋人类乳腺癌组织,左图为对照肽段,右图为抗原特异性肽段,检测抗体为Phospho-Cyclin E (Thr62) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, treated with 10 mM LiCl for the indicated times, using Phospho-Cyclin E1 (Thr62) Antibody (upper) or a cyclin E antibody (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated or hydroxyurea-treated, using Phospho-Cyclin E1 (Thr62) Antibody.


Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2 (1,2). Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7). Cyclin E1和Cyclin E2协同并激活CDK2(1)。DNA损伤时,上调/激活CDK抑制基因p21 Waf1/Cip1 and p27 Kip1能够阻滞cyclin E/CDK2的激活,从而导致G1/S期停滞。当条件适宜于细胞周期连续进行时,cyclin D/CDK4/6使Rb磷酸化从而能够降低p21 Waf1/Cip1 和 p27 Kip1的活性,使得cyclin E/CDK2随后激活(1,2)。Cyclin E/CDK2进一步磷酸化Rb使细胞周期进入S期,在此期内cyclin E/CDK2被认为可以磷酸化并激活涉及DNA合成过程的多种蛋白(2,3)。周期蛋白E的功能转化主要受磷酸化控制,这种磷酸化能够导致SCFFbw7介导的泛素化和蛋白酶体依赖的降解(4,5)。Cyclin E1在体内可以在包括苏氨酸62位,丝氨酸88位,丝氨酸72位,苏氨酸380位和丝氨酸384位的多位点磷酸化,这个磷酸化过程至少可以被两个激酶-CDK2和GSK-3调控。

  1. Lauper, N. et al. (1998) Oncogene 17, 2637-43.
  2. Lundberg, A.S. and Weinberg, R.A. (1998) Mol Cell Biol 18, 753-61.
  3. Ewen, M.E. (2000) Genes Dev 14, 2265-70.
  4. Won, K.A. and Reed, S.I. (1996) EMBO J 15, 4182-93.
  5. Koepp, D.M. et al. (2001) Science 294, 173-7.
  6. Welcker, M. et al. (2003) Mol Cell 12, 381-92.
  7. Ye, X. et al. (2004) J Biol Chem 279, 50110-9.

Application References

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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