Cell Signaling Technology

Product Pathways - Adhesion

SLK Antibody #41255

No. Size Price
41255S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
41255 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 220 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

SLK antibody recognizes endogenous levels of total SLK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu431 of human SLK protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SLK Antibody.

IP

IP

Immunoprecipitation of SLK protein from HCC1419 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SLK Antibody. Western blot analysis was performed using SLK Antibody.

Background

SLK (Ste20-like Kinase) is a member of the germinal center kinase (GCK) family of proteins. SLK has a kinase domain located at the N terminus (1). The autophosphorylation of SLK at Thr183 and Ser189 is required for the upregulation of SLK kinase activity (1, 2). The protein also has a caspase cleavage site DXXD and a SH3 binding site PXXP located in the middle part of its sequence, and a regulatory C terminal coiled-coil domain for homodimerization and adaptor binding (1-4). SLK plays important roles in development, tissue regeneration and cancer cell migration by regulating several signaling pathways (5-7). SLK phosphorylates and activates ASK1 to induce downstream p38 phosphorylation and apoptosis (8,9). During cell cycle, SLK phosphorylates Polo-like kinase (PLK) at Thr210 to promote G2/M transition (10,11). SLK also promotes cell division by direct phosphorylation of ERMs and dynactin to activate microtubule reorganization and spindle orientation (12, 13). During focal adhesion and cell migration process, SLK is activated and colocalized to the focal adhesion complex where it promotes complex turnover by phosphorylating paxillin at Ser250 (14, 15).

  1. Al-Zahrani, K.N. et al. (2013) Cell Adh Migr 7, 1-10.
  2. Luhovy, A.Y. et al. (2012) J Biol Chem 287, 5446-58.
  3. Delarosa, S. et al. (2011) Am J Physiol Renal Physiol 301, F554-64.
  4. Baron, K.D. et al. (2015) Biochim Biophys Acta 1853, 1683-92.
  5. Al-Zahrani, K.N. et al. (2014) Dev Dyn 243, 640-51.
  6. Storbeck, C.J. et al. (2013) Skelet Muscle 3, 16.
  7. Roovers, K. et al. (2009) Oncogene 28, 2839-48.
  8. Hao, W. et al. (2006) J Biol Chem 281, 3075-84.
  9. Sabourin, L.A. et al. (2000) Mol Cell Biol 20, 684-96.
  10. Ellinger-Ziegelbauer, H. et al. (2000) Genes Cells 5, 491-8.
  11. Johnson, T.M. et al. (2008) Biochemistry 47, 3688-96.
  12. Zhapparova, O.N. et al. (2013) Mol Biol Cell 24, 3205-14.
  13. Machicoane, M. et al. (2014) J Cell Biol 205, 791-9.
  14. Wagner, S. et al. (2008) PLoS One 3, e1868.
  15. Quizi, J.L. et al. (2013) Oncogene 32, 4656-63.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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