Product Pathways - PI3K / Akt Signaling
Phospho-Tuberin/TSC2 (Ser664) (D3B9Z) Rabbit mAb #40729
|40729S||100 µl ( 10 western blots )||￥4,050.00||现货查询 购买询价 防伪查询|
|40729||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
Phospho-Tuberin/TSC2 (Ser664) (D3B9Z) Rabbit mAb recognizes endogenous levels of Tuberin/TSC2 protein only when phosphorylated at Ser664. This antibody cross-reacts with a 140kD protein of unknown origin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser664 of human Tuberin/TSC2 protein.
Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).
p44/42 MAPK (Erk1/2) phosphorylates of TSC2 at Ser664 which leads to TSC1-TSC2 dissociation and considerably decreases the ability of TSC2 to inhibit mTOR signaling, cell proliferation and oncogenic transformation (8,9). Furthermore, studies have indicated that cancer patients with TSC2 phosphorylation at Ser664 may benefit from MAPK and mTOR inhibitors (10).
- Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8.
- Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9.
- Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
- Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21.
- Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70.
- Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67.
- Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
- Ma, L. et al. (2005) Cell 121, 179-93.
- Ballif, B.A. et al. (2005) Proc Natl Acad Sci U S A 102, 667-72.
- Ma, L. et al. (2007) Cancer Res 67, 7106-12.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 12630 SignalFire™ Plus ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 13038 Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb
- 13953 Prestained Protein Marker, Broad Range (11-190 kDa)
- 3612 Tuberin/TSC2 Antibody
- 3617 Phospho-Tuberin/TSC2 (Thr1462) (5B12) Rabbit mAb
- 3635 Tuberin/TSC2 (28A7) Rabbit mAb
- 3990 Tuberin/TSC2 (D57A9) Rabbit mAb
- 4060 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb
- 4308 Tuberin/TSC2 (D93F12) XP® Rabbit mAb
- 4691 Akt (pan) (C67E7) Rabbit mAb
- 5584 Phospho-Tuberin/TSC2 (Ser1387) Antibody
- 6883 SignalFire™ ECL Reagent
- 7071 Phototope-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7075 Anti-biotin, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
用户评论 --- 共 0 条