Cell Signaling Technology

Product Pathways - Protein Stability

PJA2 Antibody #40180

E3 Ligases   E3 Ubiquitin Ligases   Neurodap1   PJA   PJA-2   PJA2   Praja-2   RNF-131   RNF131   Ubiquitin Ligases  

No. Size Price
40180S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
40180 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 150 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

PJA2 Antibody recognizes endogenous levels of total PJA2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly332 of human PJA2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using PJA2 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human PJA2 protein (hPJA2-Myc/DDK; +), using PJA2 Antibody.

IP

IP

Immunoprecipitation of PJA2 from 293T cell extracts. Lane 1 is 10% input, lane 2 is immunoprecipitation with Normal Rabbit IgG #2729, and lane 3 is immunoprecipitation with PJA2 Antibody. Western blot analysis was performed with PJA2 Antibody. Anti-Rabbit HRP-conjugated light chain-specific secondary antibody was used for detection.

Background

Praja-2 (PJA2/RNF131) is a RING-H2-type E3 ubiquitin ligase. PJA2 is highly expressed in neural tissue and research studies have shown that PJA2 plays a role modulating synaptic archictecture and long-term memory by promoting the ubiquitin-dependent proteolysis of NOGO-A and PKA regulatory subunits (1,2). Research studies have also shown that PJA2 sustains gliobastoma growth by driving the ubiquitin-dependent proteasomal degradation of MOB, which inactivates the LATS tumor suppressor kinase of the Hippo pathway (3).

  1. Sepe, M. et al. (2014) Proc Natl Acad Sci U S A 111, 15729-34.
  2. Lignitto, L. et al. (2011) Nat Cell Biol 13, 412-22.
  3. Lignitto, L. et al. (2013) Nat Commun 4, 1822.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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