Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-ALK (Tyr1278/1282/1283) Antibody #3983

Anaplastic lymphoma kinase   CD246  

No. Size Price
3983S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3983T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
3983 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 80 (NPM-ALK), 220 (ALK) Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-ALK (Tyr1278/1282/1283) Antibody detects ALK only when phosphorylated at Tyr1278/1282/1283, which is equivalent to Tyr338/342/343 of NPM-ALK. This antibody might also have slight reactivity toward ALK when it is phosphorylated at Tyr1283 alone. This antibody also reacts with leukocyte tyrosine kinase (LTK) phosphorylated at Tyr672/676/677.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1278/1282/1283 of human ALK protein. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体通过合成磷酸化多肽免疫动物得到,该多肽是根据人的ALK蛋白Tyr1278/1282/1283 附近的氨基酸序列合成的。抗体经过protein A和肽亲和层析。

Western Blotting

Western Blotting

Western blot analysis of extracts from KARPAS-299 cells, untreated or treated with calf intestinal phosphatase (CIP), using Phospho-ALK (Tyr1278/1282/1283) Antibody (upper) and ALK (C26G7) Rabbit mAb #3333 (lower).

用抗磷酸化的ALK (Tyr1278/1282/1283)的抗体 (上) 或抗ALK(C26G7)兔源单克隆抗体 #3333(下)对经过或未经小牛肠碱性磷酸酶(CIP)处理的Karpas-299细胞提取物进行蛋白质印记检测。


Anaplastic lymphoma kinase (ALK) is a tyrosine kinase receptor for pleiotrophin (PTN), a growth factor involved in embryonic brain development (1-3). In ALK-expressing cells, PTN induces phosphorylation of both ALK and the downstream effectors IRS-1, Shc, PLCγ, and PI3 kinase (1). ALK was originally discovered as an NPM (nucleophosmin)-ALK fusion protein produced by a translocation (4). The NPM-ALK fusion protein is a constitutively active, oncogenic tyrosine kinase associated with anaplastic lymphoma (4). Activation of PLCγ by NPM-ALK has been suggested to be a crucial step for its mitogenic activity and may be important in the pathogenesis of anaplastic lymphomas (5). A distinct ALK oncogenic fusion protein involving ALK and EML4 has been described from a non-small cell lung cancer cell line, with corresponding fusion transcripts present in some cases of lung adenocarcinoma. The short, amino-terminal region of the microtubule-associated protein EML4 is fused to the kinase domain of ALK (6,7).

间变性淋巴瘤激酶(ALK)是多效生长因子(PTN)的酪氨酸激酶受体,它是大脑胚胎发育过程中的生长因子(1-3)。 在表达ALK的细胞中,PTN诱导ALK及其下游效应因子IRS-1, Shc, PLCγ, 和PI3K激酶发生磷酸化(1)。ALK最初是从易位突变产生的NPM-ALK融合蛋白而被发现的(4)。NPM-ALK融合蛋白是一个组成性活化,有原癌基因活性的间变性淋巴瘤相关酪氨酸激酶(4)。 由NPM-ALK介导的PLCγ的活化可能对有丝分裂活性起到关键作用,并且可能对间变性淋巴瘤的发病过程很重要(5)。另一个完全不同的ALK原癌融合蛋白在非小细胞肺癌细胞株中被报道,该蛋白融合了ALK与EML4,其相应融合转录产物在一些肺腺癌中也有表达。在EML4-ALK融合蛋白中,微管相关蛋白EML4的短氨基末端区域和ALK的激酶活性区域融合在一起(6,7)。

Phosphorylation of ALK on Tyr1278/Tyr1282/Tyr1283 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery. Phosphorylation of ALK at these three sites was observed in select carcinoma cell lines and in tumors (6).

ALK的Tyr1278/Tyr1282/Tyr1283 位点磷酸化是Cell Signaling Technology (CST)公司利用LC-MS/MS平台PhosphoScan®技术在寻找磷酸化位点过程中发现的。ALK的Tyr1278/Tyr1282/Tyr1283 位点磷酸化在某些肿瘤及肿瘤细胞系中存在(6)。

  1. Stoica, G.E. et al. (2001) J Biol Chem 276, 16772-9.
  2. Iwahara, T. et al. (1997) Oncogene 14, 439-49.
  3. Morris, S.W. et al. (1997) Oncogene 14, 2175-88.
  4. Morris, S.W. et al. (1994) Science 263, 1281-4.
  5. Bai, R.Y. et al. (1998) Mol Cell Biol 18, 6951-61.
  6. Rikova, K. et al. (2007) Cell 131, 1190-203.
  7. Takeuchi, K. et al. (2008) Clin Cancer Res 14, 6618-24.
  8. Soda, M. et al. (2007) Nature 448, 561-6.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :