Cell Signaling Technology

Product Pathways - Protein Stability

Ubiquitin (P4D1) Mouse mAb #3936

general   motif   sc-8017   ub  

No. Size Price
3936S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
3936T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价 防伪查询
3936 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Mouse IgG1
IHC-P 1:375

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Ubiquitin (P4D1) Mouse mAb detects ubiquitin, polyubiquitin and ubiquitinated proteins. This antibody may cross-react with recombinant NEDD8. Species Reactivity: All Species Expected.

Ubiquitin (P4D1)鼠单抗识别单泛素、多泛素和泛素化蛋白,这一抗体可能会与重组NEDD8发生交叉反应。 种属特异性:所有种属。

Source / Purification

Monoclonal antibody is produced by immunizing animals with 1-76 full length bovine ubiquitin.


Western Blotting

Western Blotting

Western blot analysis of 293 and HeLa cells, untreated or treated with the 26S proteasome inhibitor MG132 (50 µM, 90 minutes), using Ubiquitin (P4D1) Mouse mAb.

使用泛素(P4D1)小鼠单克隆抗体对未经或经26S蛋白酶体抑制剂MG132处理(50 µM,90分钟)的293和HeLa细胞裂解物的免疫印迹分析结果。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded breast carcinoma showing nuclear and cytoplasmic ubiquitin localization, using Ubiquitin (P4D1) Mouse mAb.



Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis (4-7).

泛素是一个在泛素——蛋白酶体途径中发挥重要作用的保守多肽。泛素能够通过泛素转移过程被共价修饰于多个细胞蛋白,导致蛋白通过26S蛋白酶体降解。三个泛素化酶参与泛素偶联过程:泛素首先与活化酶E1形成硫酯键复合体而被激活,然后被转移到泛素载体蛋白E2,然后由E2转移到E3并连接到靶蛋白赖氨酸的epsilon位氨基上(1-3)。泛素——蛋白酶体途径参与多种生物过程和疾病发生,多种信号通路蛋白如IκB、p53、cdc25a 和Bcl-2都受到泛素——蛋白酶体途径调节,影响细胞周期进程、分化、细胞应激和凋亡等过程(4-7)。

  1. Ciechanover, A. (1998) EMBO J 17, 7151-60.
  2. Hochstrasser, M. (2000) Nat Cell Biol 2, E153-7.
  3. Hochstrasser, M. (2000) Science 289, 563-4.
  4. Bernardi, R. et al. (2000) Oncogene 19, 2447-54.
  5. Aberle, H. et al. (1997) EMBO J 16, 3797-804.
  6. Salomoni, P. and Pandolfi, P.P. (2002) Nat Cell Biol 4, E152-3.
  7. Jesenberger, V. and Jentsch, S. (2002) Nat Rev Mol Cell Biol 3, 112-21.

Application References

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