Product Pathways - Cytoskeletal Signaling
Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb #39359
|39359S||100 µl ( 10 western blots )||￥4,050.00 现货查询||购买询价|
|39359||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb recognizes endogenous levels of keratin 20 protein only when phosphorylated at Ser13.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser13 of human keratin 20 protein.
Confocal immunofluorescent analysis of HT-29 cells, untreated (left), treated with Anisomycin #2222 (25 μg/mL, 30 minutes; center), or treated with anisomycin followed by λ phosphatase (right), using Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of extracts from of HT-29 cells, untreated (-), treated with Anisomycin #2222 (25 μg/ml, 30 min; +), or treated with Anisomycin and Calf Intestinal Phosphatase (CIP)/λ phosphatase (+), using Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb (upper), Keratin 20 (D9Z1Z) XP® Rabbit mAb #13063 (middle) or β-Actin (D6A8) Rabbit mAb #8547 (lower).
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
Keratin 20 is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel cells (7). Research studies have shown that keratin 20 is an important marker of colon, liver, pancreatic, Merkel cell, and gastric cancer (8). Serine 13 of keratin 20 is phosphorylated in response to stress in intestinal epithelia, likely through the p38 MAPK pathway (9, 10).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
- Moll, R. et al. (1992) Am J Pathol 140, 427-47.
- Karantza, V. (2011) Oncogene 30, 127-38.
- Zhou, Q. et al. (2006) J Biol Chem 281, 16453-61.
- Menon, M.B. et al. (2010) J Biol Chem 285, 33242-51.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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