Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb #39359

No. Size Price
39359S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
39359 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 48 Rabbit IgG
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb recognizes endogenous levels of keratin 20 protein only when phosphorylated at Ser13.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser13 of human keratin 20 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HT-29 cells, untreated (left), treated with Anisomycin #2222 (25 μg/mL, 30 minutes; center), or treated with anisomycin followed by λ phosphatase (right), using Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from of HT-29 cells, untreated (-), treated with Anisomycin #2222 (25 μg/ml, 30 min; +), or treated with Anisomycin and Calf Intestinal Phosphatase (CIP)/λ phosphatase (+), using Phospho-Keratin 20 (Ser13) (D9M6O) Rabbit mAb (upper), Keratin 20 (D9Z1Z) XP® Rabbit mAb #13063 (middle) or β-Actin (D6A8) Rabbit mAb #8547 (lower).

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

Keratin 20 is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel cells (7). Research studies have shown that keratin 20 is an important marker of colon, liver, pancreatic, Merkel cell, and gastric cancer (8). Serine 13 of keratin 20 is phosphorylated in response to stress in intestinal epithelia, likely through the p38 MAPK pathway (9, 10).

  1. Moll, R. et al. (1982) Cell 31, 11-24.
  2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
  3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
  4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
  5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
  6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
  7. Moll, R. et al. (1992) Am J Pathol 140, 427-47.
  8. Karantza, V. (2011) Oncogene 30, 127-38.
  9. Zhou, Q. et al. (2006) J Biol Chem 281, 16453-61.
  10. Menon, M.B. et al. (2010) J Biol Chem 285, 33242-51.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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