Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) #3929

4E-BP1   4ebp   BP1   PHAS-1  

No. Size Price
3929S 100 µl ( 10 western blots ) ¥4,060.00 现货查询 购买询价 防伪查询
3929 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey,D. melanogaster, Endogenous 15 to 20 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at analagous sites.

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb兔单抗检测仅在Thr37或Thr46位点磷酸化的内源性4E-BP1蛋白水平。该抗体可以与在等效位点磷酸化的4E-BP2和4E-BP3蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1.

通过人工合成小鼠4E-BP1 蛋白Thr37和Thr46位点周围序列相应的多肽片段去免疫动物从而制备单克隆抗体。


This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855.

此Cell Signaling Technology公司的抗体在最优条件接合生物素。这个抗体与未接合的Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb兔单抗 #2855具有同样的物种的交叉反应活性。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, serum-starved and stimulated with insulin (100 nM for 5 minutes), with (+) or without (-) λ phosphatase treatment, using Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) (upper) and 4E-BP1 (53H11) Rabbit mAb #9644 (lower).

使用Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (Biotinylated) (上图)和4E-BP1 (53H11) Rabbit mAb兔单抗 #9644 (下图),Western Blot分析在HeLa细胞系中磷酸化4E-BP1和4E-BP1蛋白的水平。细胞在无血清饥饿培养后再用胰岛素刺激(100 nM,5分钟),然后分为λ磷酸酶处理组(+)和未处理组。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (green) or LY294002, Wortmannin and U0126-treated (blue), using Phospho-4E-BP1 (Thr36/46) (236B4) Rabbit mAb (Biotinylated).

使用Phospho-4E-BP1 (Thr36/46) (236B4) Rabbit mAb兔单抗 (Biotinylated),流式细胞术分析Jurkat细胞,细胞分为未处理组(绿色)和LY294002, Wortmannin 和U0126处理组(蓝色)。


Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

翻译抑制蛋白4E-BP1 (又称 PHAS-1)通过结合翻译起始因子eIF4E蛋白从而抑制帽子结构依赖的翻译。4E-BP1的过磷酸化干扰上述结合并且导致激活帽子结构依赖的翻译(1)。PI3 kinase/Akt通路和FRAP/mTOR激酶通路都调节4E-BP1蛋白的激活(2,3)。在体内4E-BP1多个位点残基被磷酸化(4)。通过FRAP/mTOR蛋白使4E-BP1在Thr37和Thr46位点磷酸化不能阻止它eIF4E结合,但这被认为是为随后在4E-BP1的Ser65和Thr70位点磷酸化进行准备(5)。

  1. Pause, A. et al. (1994) Nature 371, 762-7.
  2. Brunn, G.J. et al. (1997) Science 277, 99-101.
  3. Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.
  4. Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.
  5. Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :