Render Target: SSR
Render Timestamp: 2025-01-21T18:36:44.462Z
Commit: 199712eb9daea12d88cc0e67894a8a09f475f8cb
XML generation date: 2024-08-01 15:24:08.489
Product last modified at: 2025-01-01T09:04:29.174Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Gα(z) Antibody #3904

Filter:
  • WB
Western Blotting Image 1: Gα(z) Antibody
Western blot analysis of extracts from various brain tissues using Gα(z) Antibody.

To Purchase # 3904S

Supporting Data

REACTIVITY H M R
SENSITIVITY Endogenous
MW (kDa) 40
SOURCE Rabbit
Application Key:
  • WB-Western Blotting 
Species Cross-Reactivity Key:
  • H-Human 
  • M-Mouse 
  • R-Rat 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Gα(z) Antibody detects endogenous levels of total Gα(z) protein.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Gα(z). Antibodies are purified using protein A and peptide affinity chromatography.

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins) consist of α, β and γ subunits and mediate the effects of hormones, neurotransmitters, chemokines, and sensory stimuli. To date, over 20 known Gα subunits have been classified into four families, Gα(s), Gα(i/o), Gα(q) and Gα(12), based on structural and functional similarities (1,2). Phosphorylation of Tyr356 of Gα(q)/Gα(11) is essential for activation of the G protein, since phenylalanine substitution for Tyr356 changes the interaction of Gα with receptors and abolishes ligand-induced IP3 formation (3).
Gα(z) stands out from other G proteins because it lacks an ADP-ribosylation consensus site for pertussis toxin, giving it a possible role in signal transduction pathways resistant to the toxin, such as phospholipase C (4). Gα(z) is phosphorylated and activated by protein kinase C (PKC) at Ser27 (5,6).

Pathways

Explore pathways related to this product.


For Research Use Only. Not For Use In Diagnostic Procedures.
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