Cell Signaling Technology

Product Pathways - Neuroscience

Human Brain-Derived Neurotrophic Factor (BDNF) #3897

Neurotrophin   NGF  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

E. coli

大肠杆菌

Description

Recombinant human BDNF was expressed in E. coli and is supplied in a lyophilized form. A greater than 96% purity was determined by reverse phase-HPLC and SDS-PAGE.

重组人脑源性神经营养因子(BDNF)在大肠杆菌中表达,以冻干形式提供。反相高效液相色谱法和SDS-PAGE测定其纯度大于96%。

Purity

> 96%

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells transfected with TrkB and treated with 50 ng/ml Brain-Derived Neurotrophic Factor (BDNF) for 5 minutes using Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb #4621 (upper) and total TrkB (80G2) Rabbit mAb (lower).

Western blot分析转染了TrkB且用50ng/ml Brain-Derived Neurotrophic Factor (BDNF)预处理5分钟的NIH/3T3细胞,使用的抗体是Phospho-TrkA (Tyr674/675)/TrkB (Tyr706/707) (C50F3) Rabbit mAb 兔单抗 #4621(上图)和total TrkB (80G2) Rabbit mAb 兔单抗(下图)。

Directions for Use

Working concentration of BDNF generally ranges from 50-100 ng/ml.

BDNF工作浓度约为50-100 ng/ml。

Background

Neurotrophins are comprised of at least four family members including NGF, BDNF, NT-3 and NT-4 and all are known to influence growth, development, differentiation and survival of neurons (1). Proneurotrophis bind to p75NTR but not to the family of Trk receptor tyrosine kinases (Trk) and following maturation, BDNF binds and activates TrkB. Trk receptors in turn activate three major signaling pathways: (a) Ras-MAPK signaling, which promotes neuronal differentiation and neurite outgrowth, (b) PI3 Kinase-Akt signaling, which promotes survival and growth of neurons, and (c) PLC-γ1-PKC signaling, which promotes synaptic plasticity (2). BDNF is a major regulator of transmission and plasticity at adult synapses. Moreover, the precursor proBDNF and the mature protein mBDNF drive opposite effects on long-term potentiation and long-term depression (3). BDNF has also been implicated in body weight regulation and activity: heterozygous BDNF knockout mice are hyperphagic, obese, and hyperactive (4).

神经营养因子是由至少四个成员组成的家族,包括NGF,BDNF,NT-3和NT-4,已知它们都能影响神经元的生长、发育、分化和存活(1)。Proneurotrophis与p75NTR结合,但却不与Trk受体络氨酸几倍家族结合成熟,BDNF与TrkB结合并能激活它。Trk受体反过来激活三大信号通路:(a)RAS-MAPK信号通路,从而促进神经细胞的分化和突起生长,(b)PI3激酶Akt信号通路,从而促进神经元的生存和生长,(c)PLC-γ1-PKC信号通路,从而促进突触可塑性(2)。BDNF是成熟突触传导和突触可塑性的主要调节者。此外,BDNF前体proBDNF和成熟蛋白mBDNF在长时程促进和长时程抑制中驱动着相反的作用(3)。BDNF还与体重及活动调节有关。杂合的BDNF敲除鼠会变得贪食、肥胖和多动(4)。

  1. Minichiello, L. and Klein, R. (1996) Genes Dev 10, 2849-58.
  2. Reichardt, L.F. (2006) Philos Trans R Soc Lond B Biol Sci 361, 1545-64.
  3. Martinowich, K. et al. (2007) Nat Neurosci 10, 1089-93.
  4. Kernie, S.G. et al. (2000) EMBO J 19, 1290-300.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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