Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human CD40 Ligand (hCD40L) #3583

CD154   CD40LG   gp39   T-BAM   TNFSF5   TRAP  

No. Size Price
3583SC 50 µg ( With Carrier ) ¥2,644.00 现货查询 购买询价 防伪查询
3583SF 50 µg ( Carrier Free ) ¥2,644.00 现货查询 购买询价 防伪查询
3583 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Source / Purification

Recombinant human CD40 Ligand (hCD40L) Met113-Leu161 (Accession #P29965) was produced in E. coli at Cell Signaling Technology.

CST在大肠杆菌细胞中表达重组的人CD40配体(hCD40L) Met113-Leu161 (Accession #P29965)蛋白。

Molecular Characterization

Recombinant hCD40L has a Met on the amino terminus and has a calculated MW of 16,184. DTT-reduced and non-reduced protein migrate as 16 kDa polypeptides. The expected amino terminus MQKGD of recombinant hCD40L was verified by amino acid sequencing.

重组的hCD40L蛋白在N-末端含有标签,其分子量据推算为16,184Da。DTT-还原和未还原的蛋白是作为一个16kDa 的多肽而迁移。重组 hCD40L蛋白的N-末端MQKGD的序列通过测序得到。

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hCD40L. All lots are greater than 98% pure.

6 μg 还原 (+) 和 非还原的(-) 重组hCD40L通过SDS-PAGE检测,纯度大于98%,所有批次的纯度均高于98%。

Bioactivity

The bioactivity of recombinant hCD40L was determined in a cell proliferation assay using human B cells. The ED50 of each lot is between 0.5-2 μg/ml.

重组蛋白hCD40L的生物活性是通过人B细胞的增殖实验确定的。每个批次的ED50在0.5-2 μg/ml之间。

Western Blotting

Western Blotting

Western blot analysis of extracts from THP-1 cells, untreated or treated with hCD40L for 15 minutes, using Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 (upper) and SAPK/JNK (56G8) Rabbit mAb #9258 (lower).Western免疫印迹。用Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb #4668 (上图) 和 SAPK/JNK (56G8) Rabbit mAb #9258 (下图) 研究未经处理的和经hCD40L处理15 min的 THP-1细胞的细胞提取液。

Bioactivity

Bioactivity

The proliferation of human B cells treated with increasing concentrations of hCD40L in the presence of 20 ng/ml human IL-4 (#8919) was assessed. After 96 hour treatment with hCD40L, cells were incubated with a tetrazolium salt and the OD450-OD650 was determined.在20 ng/ml hIL-4 (#8919) 存在下用递增浓度的hCD40L处理人B细胞并观察其增值能力。用hCD40L处理96小时后,细胞用四唑盐孵育并测定 OD450-OD650 数值。

Coomassie Gel

Coomassie Gel

The purity of recombinant hCD40L was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hCD40L and staining overnight with Coomassie Blue.重组hCD40L 蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组hCD40L 蛋白跑SDS胶并用考马斯亮蓝染色过夜。

Endotoxin

Less than 0.01 ng endotoxin/1 μg hCD40L.

每微克hCD40L内毒素含量小于0.01 ng。

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hCD40L. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

有载体: 每微克hCD40L蛋白溶解在包含20 μg BSA的PBS(pH 7.2)溶液中,通过 0.22 μm 滤膜并冻干。

无载体:每微克hCD40L蛋白溶解在PBS(pH 7.2)溶液中,通过 0.22 μm 滤膜并冻干。

Background

CD40 Ligand (CD40L), a member of the TNF superfamily of ligands, is expressed as either a membrane-bound or soluble homotrimer (1). Both membrane-bound and soluble forms of CD40L have biological activity (1,2). CD40L is expressed primarily on activated T cells; however, mast cells, basophils, and NK cells may also express CD40L (1). CD40L functions as an important T cell co-stimulatory molecule and enhances T-dependent B cell responses (1,2). CD40L binds CD40, which is a receptor expressed on B cells, dendritic cells, and macrophages (1). Binding of CD40L to CD40 leads to the recruitment of TRAF proteins (TRAFs 2,3,5, and 6) resulting in the activation of the JNK and NF-κB pathways (1,2).

CD40配体(CD40L),是TNF超家族配体中的一位成员,作为膜结合或可溶性的三聚体而表达(1)。CD40L的膜结合和可溶性的三聚体都具有生物活性(1,2)。CD40L主要是在激活的T细胞上表达,然而,肥大细胞、噬碱细胞和NK细胞也能表达CD40L(1)。CD40L的作用主要是T细胞的共刺激因子并增强T依赖的B细胞的应答(1,2)。CD40L结合到CD40,是作为受体表达在B细胞、树突状细胞和巨噬细胞上(1)。CD40L结合到CD40上导致了TRAF蛋白(TRAFs 2,3,5 和 6)的招募并使得JNK和 NF-κB通路激活(1,2)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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