Cell Signaling Technology

Product Pathways - Neuroscience

CRMP-2 (D8L6V) Rabbit mAb #35672

No. Size Price
35672S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
35672 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 60-80 Rabbit IgG
IP 1:50
IF-IC 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

CRMP-2 (D8L6V) Rabbit mAb recognizes endogenous levels of total CRMP-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding lle456 of human CRMP-2 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of SH-SY5Y (upper) and NCI-H522 (lower) cells using CRMP-2 (D8L6V) Rabbit mAb (green). Actin filaments have been labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissues using CRMP-2 (D8L6V) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of CRMP-2 from SH-SY5Y extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CRMP-2 (D8L6V) Rabbit mAb. Western blot analysis was performed using CRMP-2 (D8L6V) Rabbit mAb.

Background

Collapsin Response Mediator Protein-2 (CRMP-2) is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5).

  1. Gu, Y. and Ihara, Y. (2000) J Biol Chem 275, 17917-20.
  2. Yoshimura, T. et al. (2005) Cell 120, 137-49.
  3. Fukata, Y. et al. (2002) Nat Cell Biol 4, 583-91.
  4. Goshima, Y. et al. (1995) Nature 376, 509-14.
  5. Cole, A.R. et al. (2004) J Biol Chem 279, 50176-80.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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