Product Pathways - Cytoskeletal Signaling
Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #35652
|35652S||100 µl ( 50 tests )||￥4,264.00 现货查询||购买询价|
|35652||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),
Species predicted to react based on 100% sequence homology: Xenopus,
Specificity / Sensitivity
Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of α-tubulin only when acetylated at Lys40. This amino acid is not conserved in β-tubulin.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic acetylpeptide corresponding to residues surrounding Lys40 of human α-tubulin.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb #5335.
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Trichostatin A #9950 (right), using Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).
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- 5063 α-Tubulin (11H10) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 5335 Acetyl-α-Tubulin (Lys40) (D20G3) XP® Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5 is a registered trademark of Biostatus Limited.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS).
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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