Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

eNOS (D8A6N) Rabbit mAb #35362

No. Size Price
35362S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
35362 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Bovine, Endogenous 140 Rabbit IgG
IHC-P 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

eNOS (D8A6N) Rabbit mAb recognizes endogenous levels of total eNOS protein. This antibody does not cross-react with iNOS or nNOS proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1190 of human eNOS protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HUVEC (left) and HeLa (right) cell pellets using eNOS (D8A6N) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using eNOS (D8A6N) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

colon:

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using eNOS (D8A6N) Rabbit mAb.

lung:

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eNOS (D8A6N) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from EA.hy.926, HeLa and HUVEC cells using eNOS (D8A6N) Rabbit mAb (upper) or β-Tubulin (D2N5G) Rabbit mAb #15115 (lower).

IHC-P (paraffin)

IHC-P (paraffin)

lung:

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eNOS (D8A6N) Rabbit mAb.

Background

Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).

  1. Fulton, D. et al. (2001) J Pharmacol Exp Ther 299, 818-24.
  2. Shaul, P.W. (2002) Annu Rev Physiol 64, 749-74.
  3. Chen, Z.P. et al. (1999) FEBS Lett 443, 285-9.
  4. Dimmeler, S. et al. (1999) Nature 399, 601-5.
  5. Fulton, D. et al. (1999) Nature 399, 597-601.
  6. Harris, M.B. et al. (2001) J Biol Chem 276, 16587-91.
  7. Thomas, S.R. et al. (2002) J Biol Chem 277, 6017-24.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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