Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-CrkII (Tyr221) Antibody #3491

No. Size Price
3491S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3491 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Hamster, Endogenous 42 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-CrkII (Tyr221) Antibody detects endogenous levels of CrkII only when phosphorylated at tyrosine 221. The antibody cross-reacts with Tyr207-phosphorylated CrkL but does not cross-react with other tyrosine-phosphorylated proteins. Phospho-CrkII (Tyr221) Antibody仅检测tyrosine 221位点磷酸化的内源性CrkII蛋白。该抗体与Tyr207位点磷酸化CrkL蛋白发生交叉反应,但是不能与其它tyrosine位点磷酸化蛋白质发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr221 of human CrkII. Antibodies are purified by protein A and peptide affinity chromatography. 通过人工合成人源CrkII蛋白Tyr221位点周围相应的磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from K562 cells, untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-CrkII (Tyr221) Antibody. 使用Phospho-CrkII (Tyr221) Antibody,免疫印迹(Western Blot)分析K562细胞系中Phospho-CrkII (Tyr221)蛋白水平,细胞分为未处理组和calf intestinal phosphatase (CIP)处理组。


CrkII, a cellular homologue of v-Crk, belongs to a family of adaptor proteins with an SH2-SH3-SH3 domain structure that transmits signals from tyrosine kinases (1). The primary function of Crk is to recruit cytoplasmic proteins in the vicinity of tyrosine kinases through SH2-phospho-tyrosine interaction. Thus, the output from Crk depends on the SH3-binding proteins, which include the C3G and Sos guanine nucleotide exchange proteins, Abl tyrosine kinase, DOCK180 and some STE20-related kinases. The variety of Crk-binding proteins indicates the pleiotropic function of Crk (2). The two CrkII SH3 domains are separated by a 54 amino acid linker region, which is highly conserved in Xenopus, chicken and mammalian CrkII proteins (3). Tyrosine 221 in this region is phosphorylated by the Abl tyrosine kinase (4), IGF-I receptor (5) and EGF receptor (6). Once Tyr221 is phosphorylated, CrkII undergoes a change in intramolecular folding and SH2-pTyr interaction, which causes rapid dissociation of CrkII from the tyrosine kinase complex (3). CrkII蛋白是一个细胞内v-Crk的同系物,该蛋白属于含有SH2-SH3-SH3结构域的受体蛋白家族,上述结构域能传递来自酪氨酸激酶的信号(1)。Crk蛋白的主要功能是通过SH2-磷酸化-酪氨酸的相互作用招募在酪氨酸激酶附近的细胞质蛋白。因此,来自Crk蛋白的输出取决于SH3结合蛋白,这包括C3G和Sos鸟嘌呤核苷酸交换蛋白、Abl酪氨酸激酶、DOCK180和一些 STE20相关激酶。Crk结合蛋白的种类表明Crk蛋白的多种功能(2)。CrkII蛋白两个 SH3结构域通过一个54氨基酸连接区被分开,这在爪蟾属、小鸡和哺乳类CrkII蛋白中高度保守(3)。在这个区域Tyrosine 221位点通过Abl酪氨酸激酶(4)、IGF-I受体(5)和EGF受体(6)磷酸化。一旦Tyr221位点被磷酸化,CrkII蛋白在分子内折叠和SH2-pTyr 相互作用方面有一个改变,这会引起CrkII蛋白从酪氨酸激酶复合物上快速分离(3)。

  1. Zvara, A. et al. (2001) Oncogene 20, 951-961.
  2. Kiyokawa, E. et al. (1997) Crit. Rev. Oncog. 8, 329-342.
  3. Rosen, S. K. et al. (1995) Nature 374, 477-479.
  4. Amoui, M. and Miller, W.T. (2000) Cell. Signal. 12, 637-643.
  5. Koval, A. P. et al. (1998) Biochem. J. 330, 923-932.
  6. Hashimoto, Y. et al. (1998) J. Biol. Chem. 273, 17186-17191.

Application References

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