Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-DARPP-32 (Ser97) (D11A5) Rabbit mAb #3401

No. Size Price
3401S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3401 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 32 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-DARPP-32 (Ser97) (D11A5) Rabbit mAb detects endogenous levels of DARPP-32 only when phosphorylated at Ser97.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser102 of human DARPP-32 protein (equivalent to Ser97 of mouse DARPP-32 protein).

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, NIH/3T3 cells, rat brain, and C6 cells using Phospho-DARPP-32 (Ser97) (D11A5) Rabbit mAb (upper) or PKA RI-α (D54D9) Rabbit mAb #5675 (lower) (left panel). The phospho-specificity of the antibody was verified by blocking with a phospho (middle panel) or nonphosphopeptide (right panel).

Western Blotting

Western Blotting

Western blot analysis of extracts from MKN-45 cells using Phospho-DARPP-32 (Ser97) (D11A5) Rabbit mAb. The phospho-specificity of the antibody was verified by treating the membrane with (+) or without (-) calf intestinal phosphatase (CIP) after western transfer.


DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at Thr34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at Thr75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological, and behavioral phenotype (3).

Drugs of abuse such as cocaine and food reinforcement learning activate the dopamine D1 receptor-signaling cascade. The downstream effector DARPP-32 is dephosphorylated at Ser97 inhibiting its nuclear export. This enables DARPP-32 to function as an inhibitor of protein phosphatase-1, increasing phosphorylation of histone H3 at Ser10. Knock-in mice bearing a DARPP-32 Ser97 to Ala (S97A) mutation demonstrate changed behavioral effects to drugs of abuse and a decreased motivation for food (4).

  1. Nishi, A. et al. (1997) J. Neurosci. 17, 8147-8155.
  2. Bibb, J.A. et al. (1999) Nature 402, 669-671.
  3. Fienberg, A.A. et al. (1998) Science 281, 838-842.
  4. Stipanovich, A. et al. (2008) Nature 453, 879-84.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :