Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

RYBP Antibody #33924

polycomb   PRC1  

No. Size Price
33924S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价
33924 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 32 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

RYBP recognizes endogenous levels of total RYBP protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro216 of human RYBP protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT and F9 cell lines using RYBP Antibody.

Background

Ring1 and YY1-binding protein (RYBP) is a widely expressed nuclear protein that functions as a modulator of Ring1A/Ring1B-dependent histone H2A monoubiquitylation (1-3). Ring1A and Ring1B proteins function as the catalytic core subunits of polyclomb repressor complex 1 (PRC1), which acts to repress gene expression in part through monoubiquitination of histone H2A on Lys119 (4). By binding to both the YY1 DNA-binding transcription factor and Ring1A/Ring1B, RYPB is able to recruit the PRC1 complex to target loci independent of prior tri-methylation of histone H3 Lys27 by the EZH2-dependent PRC2 complex (2,3). RYBP also binds monoubiquitinated H2A Lys119 and may act to stabilize or spread binding of PRC1 across large domains of repressed chromatin (5). In addition, RYBP directly stimulates the ubiquitination activity of Ring1A/Ring1B and is required for proper differentiation of stem cells along multiple cell lineages (2,3,6,7). RYBP has also been shown to bind MDM2 and block ubiquitination and degradation of p53, leading to cell cycle arrest and apoptosis in response to DNA damage (8). Many studies demonstrate that RYBP functions as a tumor suppressor protein. RYBP expression is decreased in multiple cancers, including non-small cell lung cancer, hepatocellular carcinoma, and glioblastoma with decreased expression correlating with metastasis and poor prognosis (8-11).

  1. García, E. et al. (1999) EMBO J 18, 3404-18.
  2. Tavares, L. et al. (2012) Cell 148, 664-78.
  3. Rose, N.R. et al. (2016) Elife 5, e18591.
  4. Cao, R. et al. (2005) Mol Cell 20, 845-54.
  5. Arrigoni, R. et al. (2006) FEBS Lett 580, 6233-41.
  6. Kovacs, G. et al. (2016) Stem Cells Int 2016, 4034620.
  7. Ujhelly, O. et al. (2015) Stem Cells Dev 24, 2193-205.
  8. Chen, D. et al. (2009) EMBO Rep 10, 166-72.
  9. Voruganti, S. et al. (2015) Cancer Lett 369, 386-95.
  10. Wang, W. et al. (2014) Oncotarget 5, 11604-19.
  11. Li, G. et al. (2013) PLoS One 8, e80970.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

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