Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

SPHK2 (D2V3G) Rabbit mAb #32346

No. Size Price
32346S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
32346 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 75 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

SPHK2 (D2V3G) Rabbit mAb recognizes endogenous levels of total SPHK2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly623 of human SPHK2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from Raji, MOLT-4, and human kidney using SPHK2 (D2V3G) Rabbit mAb.

Background

Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Together with other sphingolipid metabolizing enzymes, SPHKs regulate the balance of the lipid mediators, ceramide, sphingosine, and S1P (1-4). Two distinct SPHK isoforms, SPHK1 and SPHK2, have been cloned and characterized (5,6). SPHK1 and SPHK2 are highly conserved and diversely expressed (7,8). The SPHKs are activated by G protein-coupled receptors, receptor tyrosine kinases, immunoglobulin receptors, cytokines, and other stimuli (9-12). The molecular mechanisms by which SPHK1 and SPHK2 are specifically regulated are complex and only partially understood.

  1. Hait, N.C. et al. (2006) Biochim Biophys Acta 1758, 2016-26.
  2. Xia, P. et al. (2000) Curr Biol 10, 1527-30.
  3. Hannun, Y.A. et al. (2001) Biochemistry 40, 4893-903.
  4. Futerman, A.H. and Riezman, H. (2005) Trends Cell Biol 15, 312-8.
  5. Kohama, T. et al. (1998) J Biol Chem 273, 23722-8.
  6. Liu, H. et al. (2000) J Biol Chem 275, 19513-20.
  7. Liu, H. et al. (2002) Prog Nucleic Acid Res Mol Biol 71, 493-511.
  8. Spiegel, S. and Milstien, S. (2003) Nat Rev Mol Cell Biol 4, 397-407.
  9. Alemany, R. et al. (2007) Naunyn Schmiedebergs Arch Pharmacol 374, 413-28.
  10. Saba, J.D. and Hla, T. (2004) Circ Res 94, 724-34.
  11. Anliker, B. and Chun, J. (2004) J Biol Chem 279, 20555-8.
  12. Wattenberg, B.W. et al. (2006) J Lipid Res 47, 1128-39.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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