Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Etk (Tyr40) Antibody #3211


No. Size Price
3211S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3211 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 76 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Etk (Tyr40) Antibody detects endogenous levels of Etk only when phosphorylated at Tyr40. This antibody may cross react with activated EGF Receptor. This antibody may cross react with other family members including ITK and BTK when overexpressed.

磷酸化Etk (Tyr40)的抗体仅在Etk的Tyr40位点磷酸化时能检测到内源Etk蛋白的表达。本抗体可能与激活的EGFR以及家族其它成员如高表达的ITK 和 BTK,发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr40 of human Etk. Antibodies are purified by protein A and peptide affinity chromatography. 多克隆抗体通过用多肽免疫动物得到,该磷酸化多肽是根据人的Etk蛋白Tyr40附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or anti-CD3 antibody-treated, using Phospho-Etk (Tyr40) Antibody (upper) or Etk Antibody #3212 (lower). 用抗磷酸化Etk (Tyr40)的抗体(上)或抗Etk 的抗体 #3212(下)对经过或未经抗CD3抗体预处理的Jurkat细胞进行免疫印迹检测。


Etk, also known as BMX, is a member of the Bruton's tyrosine kinase (Btk) family (1). It is expressed in a variety of hematopoietic, epithelial and endothelial cells. Etk, like other Btk family members, contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. It participates in multiple signal transduction pathways (2). Phosphorylation of Tyr566 by Src kinase is required for activation of Etk in vivo (3). In endothelial and epithelial cells, Etk is regulated by FAK through phosphorylation at Tyr40 (4).

Etk,即BMX,是Bruton's 酪氨酸激酶(Btk)家族的成员之一(1)。它表达在各种造血细胞、上皮细胞和内皮细胞中。Etk和Btk家族的其它成员一样含有一个普列克底物蛋白同源性 (PH)区以及Src 同源性 SH3和 SH2区。它参与到多种信号转导途径(2)。Src激酶使Tyr566磷酸化对于体内Etk的活化是必须的(3)。在内皮细胞和上皮细胞中,FAK 通过Tyr40 位点的磷酸化调控Etk(4)。

  1. Tamagnone, L. et al. (1994) Oncogene 9, 3683-8.
  2. Qiu, Y. and Kung, H.J. (2000) Oncogene 19, 5651-61.
  3. Tsai, Y.T. et al. (2000) Mol Cell Biol 20, 2043-54.
  4. Chen, R. et al. (2001) Nat Cell Biol 3, 439-44.

Application References

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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