Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Ack1 (Tyr284) Antibody #3138

No. Size Price
3138S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
3138 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 135 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-Ack1 (Tyr284) Antibody detects transfected levels of Ack1 only when phosphorylated at Tyr284.

Phospho-Ack1 (Tyr284) 抗体仅识别转染水平的Tyr284磷酸化的Ack1蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr284 of human Ack1. Antibodies are purified by peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or overexpressing Myc-tagged Ack1 protein, using Phospho-Ack1 (Tyr284) Antibody (upper and middle) and Myc-Tag (71D10) Rabbit mAb #2278 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. (The lysates of COS cells overexpressing Myc-tagged Ack1 were kindly provided by Dr. Wannian Yang, Weis Center for Research, Geisinger Clinic.) 使用Phospho-Ack1 (Tyr284) Antibody (上图和中图)和Myc-tag (71D10) Antibody #2278 (下图),免疫印迹(Western Blot)分析在COS细胞系中未转染、转染的Myc标记的人源Ack1表达质粒。中图在抗体检测之前使用calf intestinal phosphatase (CIP)处理。(转染Myc标记的人源Ack1表达质粒的COS细胞裂解液是由Dr. Wannian Yang, Weis Center for Research, Geisinger Clinic友好提供。)


Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region (1,2). Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF and PDGF, as well as by activated integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility (3,4). Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer (5).

Ack1和Ack2 (activated cdc42-associated kinase 1 and 2)蛋白是非受体酪氨酸激酶,由一个酪氨酸激酶核心、一个SH3区域、一个Cdc42/Rac相互作用粘附区(CRIB)、一个 Ralt 同源区域和一个脯氨酸富足区域构成(1,2)。Ack1和Ack2是仅有的两个已知的与cdc42相互作用的酪氨酸激酶。通过生长因子包括EGF和PDGF以及细胞粘附分子包括活化的整合素可能激活两个Ack蛋白 ,Ack蛋白可能作用是联系酪氨酸激酶受体或与cdc42相关的G蛋白偶联受体信号。Acks 可能调节细胞生长、形态和动力(3,4)。最近研究发现指出Ack1蛋白可能在前列腺肿瘤的发生起着重要作用,这能使它对于这种类型癌症作为一个潜在药物靶点(5)。

Tyr284 is located in the activation loop of Ack1 and is a primary autophosphorylation site critical for Ack1 kinase activity (5).


  1. Galisteo, M.L. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 9796-9801.
  2. Yokoyama, N. and Miller, W.T. (2003) J. Biol. Chem. 278, 47713-47723.
  3. Yang, W. and Cerione, R.A. (1997) J. Biol. Chem. 272, 24819-24824.
  4. Yang, W. et al. (2001) J. Biol. Chem. 276, 43987-43993.
  5. Mahajan, N.P. et al. (2005) Cancer Res. 65, 10514-10523.
  6. Yokoyama, N. and Miller, W.T. (2006) Methods Enzymol. 406, 250-260.

Application References

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