Cell Signaling Technology

Product Pathways - Metabolism

IGFBP1 (D4E9T) XP® Rabbit mAb #31025

No. Size Price
31025S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价 防伪查询
31025T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价 防伪查询
31025 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 30 Rabbit IgG
IHC-P 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

IGFBP1 (D4E9T) Rabbit mAb recognizes endogenous levels of total IGFBP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro240 of human IGFBP1 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HepG2 (left) and SKMEL28 (right) cell pellets using IGFBP1 (D4E9T) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human liver with chronic inflammation using IGFBP1 (D4E9T) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human decidual cells of the placenta using IGFBP1 (D4E9T) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using IGFBP1 (D4E9T) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IGFBP1 (D4E9T) Rabbit mAb.

Background

Insulin-like growth factor-binding proteins (IGFBPs) play an integral role in modifying insulin-like growth factor (IGF) actions in a wide variety of cell types. There are six known IGFBP family members (IGFBP1-6), which are structurally related, but encoded by distinct genes. IGFBPs have high affinity for IGFs; in some contexts, IGFBPs inhibit IGF actions by preventing access to IGF receptors, while in others they potentiate IGF actions by facilitating ligand-receptor interaction (1-3). IGFBP1 is produced primarily by the liver and secreted into circulation, and studies show its expression can be negatively regulated by insulin (4, 5). Notably, low levels of IGFBP1 were shown to predict the future onset of Type 2 diabetes (5). Reduced expression of IGFBP1 expression was also associated with tumor progression in breast cancer, prostate cancer, pancreatic cancer and colorectal cancer, possibly stemming from reduced inhibition of mitogenic IGF signaling (6-9). Notably however, other research studies have reported increased levels of IGFBP1 in selected tumor types; in human schwannoma, increased IGFBP1 was associated with stimulation of the integrin β1/FAK pathway, supporting the concept of IGF-independent signaling functions for selected IGFBPs (10,11).

  1. Duan, C. (2002) J Endocrinol 175, 41-54.
  2. Sandhu, M.S. et al. (2002) J Natl Cancer Inst 94, 972-80.
  3. Baxter, R.C. (2014) Nat Rev Cancer 14, 329-41.
  4. Ross, R.J. et al. (1994) J Endocrinol 141, 377-82.
  5. Lewitt, M.S. et al. (2014) J Clin Med 3, 1561-74.
  6. Park, J.H. et al. (2015) Neoplasia 17, 421-33.
  7. Wolpin, B.M. et al. (2007) Cancer Res 67, 7923-8.
  8. Cao, Y. et al. (2015) Int J Cancer 136, 2418-26.
  9. Purandare, S.M. et al. (2009) Am J Med Genet A 149A, 1740-8.
  10. Ammoun, S. et al. (2012) Oncogene 31, 1710-22.
  11. Wheatcroft, S.B. et al. (2009) Trends Endocrinol Metab 20, 153-162.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

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Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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