Product Pathways - Tyrosine Kinase / Adaptors
Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb #3098
|3098S||100 µl ( 10 western blots )||￥4,050.00 现货查询||购买询价|
|3098||carrier free & custom formulation / quantity||email request|
|W||1:1000||Human,||Endogenous||135 (c-Abl); 210 (Bcr-Abl)||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Species predicted to react based on 100% sequence homology: Mouse,
Specificity / Sensitivity
Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb detects endogenous levels of c-Abl only when phosphorylated at Tyr89. This antibody may cross-react with other tyrosine-phosphorylated proteins.
Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb兔单抗能检测内源性Tyr89位点磷酸化的c-Abl蛋白。这种抗体可能会与其他酪氨酸位点磷酸化的蛋白发生交叉反应。
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr89 of human c-Abl.
Western blot analysis of extract from CML-T1 leukemic cells with Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb (upper and middle) or c-Abl Antibody #2862 (lower). The phospho-specificity of this rabbit mAb was verified by treating the membrane with calf intestinal phosphatase (CIP) (middle and lower) before antibody probing.western blot方法检测细胞提取物：用Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb (上图和中图)和c-Abl Antibody #2862 (下图)处理的CML-T1白血病细胞。在使用抗体检测之前，先用牛小肠碱性磷酸酶处理转印膜已验证这种特异位点磷酸化的兔单克隆抗体。
The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).
c-Abl原癌基因编码一个非受体蛋白酪氨酸激酶，该激酶在多细胞动物中普遍表达且高度保守。c-Abl蛋白分布于胞核以及胞质中，参与调节细胞增殖、分化、凋亡细胞粘附和应激反应(1-3)。在体内，通过整合素激活、PDGF刺激以及结合到c-Jun、Nck 和RFX1 蛋白上(2,4)等各种生理刺激，能使c-Abl激酶的活力提高。调节c-Abl蛋白激酶活力的体内机制目前尚不完全清楚。Tyr245位点位于SH2和催化区之间的连接区域，Abl蛋白家族在这个位点处都是保守的。Tyr245位点的磷酸化涉及到c-Abl激酶的活化（5）。另外，位于Abl蛋白的激酶活化环处的Tyr412位点，对激酶的活性也是必需的（6）。
Phosphorylation of c-Abl on Tyr89 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery as well as another publication using MS technology (7). For additional information please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org.
- Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
- Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.
- Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
- Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.
- Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
- Pluk, H. et al. (2002) Cell 108, 247-259.
- Meyn, M.A. et al. (2006) J. Biol. Chem. 281, 30907-30916.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
U.S. Patent No. 5,675,063.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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