Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Tau (Ser199) Antibody #29957

No. Size Price
29957S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
29957 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 50-80 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-Tau (Ser199) Antibody

recognizes endogenous levels of Tau protein only when phosphorylated at Ser199. This antibody also detects a 110 kDa band of unknown origin.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser199 of human Tau protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using Phospho-Tau (Ser199) Antibody. The phospho-specificity of Phospho-Tau (Ser199) Antibody was verified by peptide blocking using a phosphopeptide or non-phosphopeptide targeting residue Ser199.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Ser199 of Tau is phosphorylated by various kinases such as GSK-3β, AMP-activated protein kinase (AMPK), and dual specificity tyrosine-phosphorylation-regulated kinase 1A (Dyrk1A) (4-7). Phosphorylation of Ser199 of Tau is an early event in the pathogenesis of Alzheimer's disease (8,9).

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
  3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
  4. Qian, W. et al. (2010) J Alzheimers Dis 19, 1221-9.
  5. Leroy, A. et al. (2010) J Biol Chem 285, 33435-44.
  6. Thornton, C. et al. (2011) Biochem J 434, 503-12.
  7. Jin, N. et al. (2015) J Biol Chem 290, 15219-37.
  8. Mondragón-Rodríguez, S. et al. (2008) Neuropathol Appl Neurobiol 34, 62-75.
  9. Mondragón-Rodríguez, S. et al. (2008) Int J Exp Pathol 89, 81-90.

Application References

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Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

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