Product Pathways - Neuroscience
Phospho-Tau (Ser199) Antibody #29957
|29957S||100 µl ( 10 western blots )||￥3,900.00 现货查询||购买询价|
|29957||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting,
Specificity / Sensitivity
Phospho-Tau (Ser199) Antibodyrecognizes endogenous levels of Tau protein only when phosphorylated at Ser199. This antibody also detects a 110 kDa band of unknown origin.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser199 of human Tau protein. Antibodies are purified by protein A and peptide affinity chromatography.
Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).
Ser199 of Tau is phosphorylated by various kinases such as GSK-3β, AMP-activated protein kinase (AMPK), and dual specificity tyrosine-phosphorylation-regulated kinase 1A (Dyrk1A) (4-7). Phosphorylation of Ser199 of Tau is an early event in the pathogenesis of Alzheimer's disease (8,9).
- Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
- Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
- Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
- Qian, W. et al. (2010) J Alzheimers Dis 19, 1221-9.
- Leroy, A. et al. (2010) J Biol Chem 285, 33435-44.
- Thornton, C. et al. (2011) Biochem J 434, 503-12.
- Jin, N. et al. (2015) J Biol Chem 290, 15219-37.
- Mondragón-Rodríguez, S. et al. (2008) Neuropathol Appl Neurobiol 34, 62-75.
- Mondragón-Rodríguez, S. et al. (2008) Int J Exp Pathol 89, 81-90.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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