Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb #2909

No. Size Price
2909S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
2909 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb detects endogenous levels of proteins containing the ATM/ATR substrate motif. This antibody preferentially binds peptides and proteins that contain phospho-Ser/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position. The antibody does not cross-react with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb兔单抗识别包含ATM/ATR底物基序的蛋白质。此抗体优先结合包含磷酸化丝氨酸/苏氨酸的肽段和蛋白质,还要求-1位有亮氨酸或相似的疏水氨基酸以及+1位是谷氨酰胺。此抗体不与相应的非磷酸化序列或其它包含磷酸化丝氨酸/苏氨酸的基序发生交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-ATM/ATR substrate peptides.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or treated with γ-irradiation, using Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb.

对未处理(-)或γ-irradiation处理的HeLa细胞抽提液使用Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb进行Western blot分析。


Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation (3). The complex phenotype of AT cells suggests that it likely has additional substrates (3). To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif.


  1. Kastan, M.B. and Lim, D.S. (2000) Nature Rev. Mol. Cell Biol. 1, 179-186.
  2. Zhao, H. and Piwnica-Worms, H. (2001) Mol. Cell. Biol. 21, 4129-4139.
  3. Kim, S. T. et al. (1999) J. Biol. Chem. 274, 37538-37543.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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