Cell Signaling Technology

Product Pathways - Metabolism

Phospho-C/EBPα (Thr222/226) Antibody #2844

C/EBP-α   C/EBPα   cebp   cebp-alpha   CHOP  

No. Size Price
2844S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
2844T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
2844 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 30, 42, 45 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Rat,

Specificity / Sensitivity

Phospho-C/EBPalpha (Thr222/226) Antibody detects endogenous levels of C/EBPalpha only when phosphorylated at threonine 222 and 226. This antibody does not cross-react with other phosphorylated C/EBP isoforms.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr222/226 of mouse C/EBPalpha. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from U937 cells treated with either LiCl or NaCl for the indicated times, using Phopho-C/EBPalpha (Thr222/226) Antibody (upper) and C/EBPalpha antibody (lower). C/EBPalpha phosphorylation at Thr222/226 is abolished by the specific GSK3 inhibitor LiCl, but not by NaCl, indicating that phosphorylation at these sites are depends on GSK3 kinase.

对U937细胞抽提液,LiCl或NaCl处理适当时间,使用Phopho-C/EBPalpha (Thr222/226) Antibody(上图)或C/EBPalpha antibody(下图)进行Western blot分析。C/EBPalpha在Thr222/226的磷酸化被特异性的GSK3抑制剂LiCl消除,但不能被氯化钠消除,这表明这些位点的磷酸化需要GSK3激酶。


CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-1 stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226 and Ser230 by GSK3 seems to be required for adipogenesis (4).

CCAAT/增强子结合蛋白(C/EBP)是一个转录因子家族,对细胞分化、终端功能和炎症反应十分关键(1)。目前已确定该家族的6个成员(C/EBPα, β, δ, γ, ε, ζ),它们分布在不同组织(1)。由不同的启动子开始转录,可以得到两种不同的C/EBPα(p42和p30),两者均是强转录激活因子(2)。有报道发现胰岛素和胰岛素样生长因子1刺激C/EBPα的去磷酸化,在胰岛素诱导的GLUT4转录抑制中扮演重要角色(3)。C/EBPα被GSK3在Thr222, Thr226, 和Ser230的磷酸化似乎在脂肪生成中必需(4)。

  1. Lekstrom-Hims, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
  2. Lin, F. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 9606-9610.
  3. Hemati, N. et al. (1997) J. Biol. Chem. 272, 25913-25919.
  4. Ross, S.E. et al. (1999) Mol. Cell. Biol. 19, 8433-8441.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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