Hip Antibody #2723
Filter:
- WB
Western blot analysis of extracts from various cell types using Hip Antibody.
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 48 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Related Products
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Hip Antibody detects endogenous levels of total Hip protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Hip. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Hip (HSP70-interacting protein), also known as ST13 (suppression of tumorigenicity protein 13), is one of several co-chaperones that regulate activities of the HSP70 chaperone family (1,2). The homo-oligomeric protein Hip cooperates with HSP70 in protein folding by stabilizing the ADP-bound state of HSP70. Hip directly binds to the ATPase domain of HSP70 when it is converted to the ADP-bound state by proteins of the HSP40 family (3). By collaborating with other positive co-factors such as HSP40 and Hop, or competing with negative co-factors such as Bag1, Hip may facilitate the chaperone function of HSP70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and various regulators of proliferation or apoptosis (4-8).
- Prapapanich, V. et al. (1996) Mol. Endocrino.l 10, 420-431.
- Gebauer, M. et al. (1997) FEBS Lett. 417, 109-113.
- Höhfeld, J. et al. (1995) Cell 83, 589-.98.
- Frydman, J. and Höhfeld, J. (1997) Trends Biochem. Sci. 22, 87-92.
- Nollen, E.A. et al. (2001) J. Biol. Chem. 276, 4677-4682.
- Fan, G.H. et al. (2002) J. Biol. Chem. 277, 6590-6597.
- Nelson, G.M. et al. (2004) Mol. Endocrinol. 18, 1620-1630.
- Shi, Z.Z. et al. (2007) J. Zhejiang Univ. Sci. B. 8, 170-176.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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