Product Pathways - Lymphocyte Signaling
BCL6 (D4I2V) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #26556
|26556S||100 µl ( 50 tests )||￥4,264.00||现货查询 购买询价 防伪查询|
|26556||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Specificity / Sensitivity
BCL6 (D4I2V) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total BCL6 protein. It is predicted to detect both human BCL6 isoform 1 and isoform 2.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment spanning residues Asp210 through Thr409 of human BCL6 protein.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated BCL6 (D4I2V) XP® Rabbit mAb #14895.
Chromosomal translocations result in misregulation of the proto-oncogene BCL6 in patients with B cell-derived non-Hodgkin's lymphoma (1). The BCL6 gene is selectively expressed in mature B cells and encodes a nuclear phosphoprotein that belongs to the BTB/POZ zinc finger family of transcription factors (2,3). BCL6 protein can bind to target DNA sequences of Stat6 and, analogous to Stat6, modulate the expression of interleukin-4-induced genes (4). Furthermore, BCL6 restrains p53-dependent senescence, making BCL6-active tumors functionally p53-negative (5). The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin/proteasome pathway in B cells (6,7). In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300 (8).
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- Harris, M.B. et al. (1999) Mol Cell Biol 19, 7264-75.
- Shvarts, A. et al. (2002) Genes Dev 16, 681-6.
- Moriyama, M. et al. (1997) Oncogene 14, 2465-74.
- Niu, H. et al. (1998) Genes Dev 12, 1953-61.
- Bereshchenko, O.R. et al. (2002) Nat Genet 32, 606-13.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS).
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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