Product Pathways - Jak/Stat Pathway
Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb (PE Conjugate) #25809
|25809S||100 µl ( 50 tests )||￥3,986.00 现货查询||购买询价|
|25809||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry,
Species predicted to react based on 100% sequence homology: Monkey,
Specificity / Sensitivity
Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb (PE Conjugate) recognizes endogenous levels of Stat1 protein only when phosphorylated at Tyr701.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr701 of human Stat1 protein.
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb #7649.
The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.
- Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
- Durbin, J.E. et al. (1996) Cell 84, 443-50.
- Meraz, M.A. et al. (1996) Cell 84, 431-42.
- Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
- Frank, D.A. (1999) Mol Med 5, 432-56.
- Wen, Z. et al. (1995) Cell 82, 241-50.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
用户评论 --- 共 0 条