Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-CDK2 (Thr160) Antibody #2561

cdk   cyclin  

No. Size Price
2561S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
2561 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 33 Rabbit
IP 1:50
F 1:25
E-P 1:2000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-CDK2 (Thr160) Antibody detects endogenous levels of CDK2 only when phosphorylated at threonine 160. The antibody weakly cross-reacts with cdc2 phosphorylated at Thr161. It does not cross-react with other phosphorylated cyclin dependent kinases. Phospho-CDK2 (Thr160) Antibody 能够检测内源性苏氨酸(160位)磷酸化的CDK2蛋白,该抗体与苏氨酸(161位)磷酸化的cdc2蛋白有轻微交叉反应,与其他磷酸化周期蛋白依赖性激酶不发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr160 of human CDK2. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对CDK2蛋白的苏氨酸(160位)磷酸化肽段免疫动物,利用蛋白A和多肽亲和层析技术生产的。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-CDK2 (Thr160) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-CDK2 (Thr160)-positive cells. 流式细胞术分析未处理的Jurkat细胞,使用的抗体为Phospho-CDK2 (Thr160) Antibody ,PI染色法检测DNA含量。框内的细胞群为phospho-CDK2 (Thr160)阳性细胞。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells synchronized in G0, G1/S or Mitosis, using Phospho-CDK2 (Thr160) Antibody.


Cyclin-dependent kinase 2 (p33CDK2) is an important component of the cell cycle machinery. Like p34cdc2, kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a CDK inhibitor. Inhibitory phosphorylation occurs on Thr14 and Tyr15 (1). Inhibition of CDK2-cyclin complexes can also be attributed to association with p27 Kip1 and p21 Waf1/Cip1 (2). Activation of CDK2 complexes requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160 (3), which is mediated by CAK, a complex of CDK7 and cyclin H (4). CDK2/cyclin E kinase activity is important for the G1 to S transition and phosphorylation of the Rb protein. During S-phase, active CDK2/cyclin A complexes predominate and phosphorylate E2F and the active CDK2 complex persists in the nucleus throughout G2 (5). 周期蛋白依赖性激酶2(p33CDK2)是细胞周期机制的重要组成部分。类似于p34cdc2,激酶活性的调节经由磷酸化状态以及周期蛋白亚基和CDK抑制剂之间的关系。磷酸化抑制通常发生在14位和15位苏氨酸(1)。CDK2-周期蛋白复合体的抑制也与p27 Kip1和 p21 Waf1/Cip1之间的关系有关(2)。CDK2复合体的磷酸化需要cdc25磷酸酶和160位苏氨酸磷酸化引(由CDK7和cyclin H组成的CAK复合体介导)起的14位和15位苏氨酸去磷酸化。 CDK2/周期蛋白E激酶活性对于G1到S期的转换和Rb蛋白的磷酸化具有重要意义。在S期,活化的CDK2/周期蛋白A复合体支配和磷酸化E2F,而活化的CDK2复合体持续在G2期的胞核中存在(5)。

  1. Morgan, D.O. (1995) Nature 374, 131-4.
  2. Poon, R.Y. et al. (1996) J Biol Chem 271, 13283-91.
  3. Gu, Y. et al. (1992) EMBO J 11, 3995-4005.
  4. Fesquet, D. et al. (1993) EMBO J 12, 3111-21.
  5. Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :