Product Pathways - Lymphocyte Signaling
PD-L1 (Extracellular Domain Specific) (D8T4X) Rabbit mAb (Alexa Fluor® 488 Conjugate) #25048
|25048S||100 µl ( 50 tests )||￥4,060.00||现货查询 购买询价 防伪查询|
|25048||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry), CS-CA=CellSimple Cell-Assay,
Specificity / Sensitivity
PD-L1 (Extracellular Domain Specific) (D8T4X) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total PD-L1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with mammalian cells expressing full length PD-L1 protein.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated PD-L1 (Extracellular Domain Specific) (D8T4X) Rabbit mAb #86744.
Confocal immunofluorescent analysis of HCC1954 (left), MDA-MB-231 (middle), and A549 (right) using PD-L1 (Extracellular Domain Specific) (D8T4X) (Alexa Fluor® 488 Conjugate) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of SUP-M2 cells (green), MDA-MB-231 cells (blue), and Ramos cells (red) using using PD-L1 (Extracellular Domain Specific) (D8T4X) Rabbit mAb (Alexa Fluor® 488 Conjugate).
Programmed cell death 1 ligand 1 (PD-L1, B7-H1, CD274) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. The PD-L1 ligand binds the PD-1 transmembrane receptor and inhibits T cell activation. PD-L1 was discovered following a search for novel B7 protein homologs and was later shown to be expressed by antigen presenting cells, activated T cells, and tissues including placenta, heart, and lung (1-3). Similar in structure to related B7 family members, PD-L1 protein contains extracellular IgV and IgC domains and a short, cytoplasmic region. Research studies demonstrate that PD-L1 is expressed in several tumor types, including melanoma, ovary, colon, lung, breast, and renal cell carcinomas (4-6). Expression of PD-L1 in cancer is associated with tumor infiltrating lymphocytes, which mediate PD-L1 expression through the release of interferon gamma (7). Additional research links PD-L1 expression to cancers associated with viral infections (8,9).
- Dong, H. et al. (1999) Nat Med 5, 1365-9.
- Freeman, G.J. et al. (2000) J Exp Med 192, 1027-34.
- Liang, S.C. et al. (2003) Eur J Immunol 33, 2706-16.
- Dong, H. et al. (2002) Nat Med 8, 793-800.
- Thompson, R.H. et al. (2006) Cancer Res 66, 3381-5.
- Pardoll, D.M. (2012) Nat Rev Cancer 12, 252-64.
- Taube, J.M. et al. (2012) Sci Transl Med 4, 127ra37.
- Lyford-Pike, S. et al. (2013) Cancer Res 73, 1733-41.
- Chen, B.J. et al. (2013) Clin Cancer Res 19, 3462-73.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 14772 PD-L1 (E1L3N®) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 35423 CellSimple™ Cassettes
- 86744 PD-L1 (Extracellular Domain Specific) (D8T4X) Rabbit mAb
- 88416 CellSimple™ Cell Analyzer
For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5 is a registered trademark of Biostatus Limited.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
The Alexa Fluor dye conjugates in this product are sold under license from Life Technologies Corporation, for research use only excluding use in combination with DNA microarrays and high content screening (HCS).
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, including use with HCS or other automated imaging applications but excluding use in combination with DNA microarrays. The buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or firstname.lastname@example.org.
CellSimple is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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