Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Etk/BMX (D6J1S) Rabbit mAb #24773

No. Size Price
24773S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
24773 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 78 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Etk/BMX (D6J1S) Rabbit mAb recognizes endogenous levels of total Etk/BMX protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Etk/BMX protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Etk/BMX (D6J1S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower) to confirm equal loading.

Background

Etk, also known as BMX, is a member of the Bruton's tyrosine kinase (Btk) family (1). It is expressed in a variety of hematopoietic, epithelial and endothelial cells. Etk, like other Btk family members, contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. It participates in multiple signal transduction pathways (2). Phosphorylation of Tyr566 by Src kinase is required for activation of Etk in vivo (3). In endothelial and epithelial cells, Etk is regulated by FAK through phosphorylation at Tyr40 (4).

  1. Tamagnone, L. et al. (1994) Oncogene 9, 3683-8.
  2. Qiu, Y. and Kung, H.J. (2000) Oncogene 19, 5651-61.
  3. Tsai, Y.T. et al. (2000) Mol Cell Biol 20, 2043-54.
  4. Chen, R. et al. (2001) Nat Cell Biol 3, 439-44.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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