Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-HSP27 (Ser78) Antibody #2405

heat shock   heat shock protein   hsp   hsp 27   hsp27   phospho-hsp  

No. Size Price
2405S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
2405T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
2405 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 27 Rabbit
IHC-P 1:100
F 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

Phospho-HSP27 (Ser78) Antibody detects endogenous levels of HSP27 phosphorylated at Ser78 . This antibody does not cross-react with other phosphorylated heat shock proteins.

Phospho-HSP27 (Ser78) Antibody识别内源性的Ser78磷酸化的HSP27。此抗体不与其它HSF蛋白相互作用。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser78 of human HSP27. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and COS cells, untreated, anisomycin-treated or UV-treated, using Phospho-HSP27 (Ser78) Antibody (upper) or HSP27 (G31) mAb #2402 (lower).

对HeLa和COS细胞抽提液,未处理或anisomycin处理或UV处理,使用Phospho-HSP27 (Ser78) Antibody(上图)或HSP27 (G31) mAb #2402(下图)进行Western blot分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-HSP27 (Ser78) Antibody.

对石蜡包埋的人乳腺癌使用Phospho-HSP27 (Ser78) Antibody进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-HSP27 (Ser78) Antibody.

对石蜡包埋的人肺癌使用Phospho-HSP27 (Ser78) Antibody进行免疫组化分析。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells, untreated (blue) or UV treated (green), using Phospho-HSP27 (Ser78) Antibody compared to a nonspecific negative control antibody (red).

使用Phospho-HSP27 (Ser78) Antibody和非特异性阴性对照抗体(红色),未处理(蓝色)或用NGF处理(绿色),对HeLa细胞进行流式细胞仪分析。


Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small heat shock proteins, HSP27 is regulated at both the transcriptional and posttranslational levels (1). In response to stress, the expression level of HSP27 increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78 and Ser82 by MAPKAP kinase 2 as a result of the activation of the p38 MAP kinase pathway (2,3). Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers (4). It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization (5,6).

热休克蛋白(HSP)27是小HSP之一,在多种细胞和组织中都有不同水平的组成性表达。像其它小HSP一样,HSP27的调节也发生在转录和翻译后(1)。应激反应中,HSP27表达增加了数倍以实现细胞对不利环境的抵抗。HSP27被MAPKAPK-2在Ser15、Ser78和Ser82磷酸化,作为p38 MAP激酶途径活化的结果(2,3)。HSP27磷酸化导致其四级结构改变,以至于从大分子量的同型多聚体变为二聚体和单体(4)。目前显示HSP27磷酸化和浓度增加可以调节肌动蛋白多聚化和再组织(5,6)。

  1. Arrigo, A.P. and Landry, J. (1994) Cold Spring Harbor Laboratory Press, NY, 335-373.
  2. Landry, J. et al. (1992) J. Biol. Chem. 267, 794-803.
  3. Rouse, J. et al. (1994) Cell 78, 1027-1037.
  4. Rogalla, T. et al. (1999) J. Biol. Chem. 274, 18947-18956.
  5. Lavoie, J. et al. (1993) J. Biol. Chem. 268, 24210-24214.
  6. Rousseau, S. et al. (1997) Oncogene 15, 2169-2177.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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