Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-Tuberin/TSC2 (Ser1387) (D2R3A) Rabbit mAb #23402

No. Size Price
23402S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
23402 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 200 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Tuberin/TSC2 (Ser1387) (D2R3A) Rabbit mAb detects endogenous levels of tuberin protein only when phosphorylated at Ser1387. This antibody may also cross-reacts with an unidentified 140 kD protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1387 of human tuberin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, untreated or phosphatase-treated, using Phospho-Tuberin/TSC2 (Ser1387) (D2R3A) Rabbit mAb (upper) or Tuberin/TSC2 (D93F12) XP® Rabbit mAb #4308 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from wild-type (wt) and TSC2-deficient (-/-) mouse embryonic fibroblasts (MEF) using

Phospho-Tuberin/TSC2 (Ser1387) (D2R3A) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Background

Tuberin is a product of the TSC2 tumor suppressor gene and an important regulator of cell proliferation and tumor development (1). Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors (2). Tuberin is directly phosphorylated at Thr1462 by Akt/PKB (3). Phosphorylation at Thr1462 and Tyr1571 regulates tuberin-hamartin complexes and tuberin activity (3-5). In addition, tuberin inhibits the mammalian target of rapamycin (mTOR), which promotes inhibition of p70 S6 kinase, activation of eukaryotic initiation factor 4E binding protein 1 (4E-BP1, an inhibitor of translation initiation), and eventual inhibition of translation (3,6,7).

Phosphorylation of tuberin by AMPK at Ser1387 is necessary for cell size control in response to energy deprivation and protects from apoptosis (8). Furthermore, phosphorylation at Ser1387 primes phosphorylation by GSK3 of upstream sites (Ser1383, Ser1379 and Ser1375), integrating Wnt signaling (9).

  1. Soucek, T. et al. (1998) Proc Natl Acad Sci U S A 95, 15653-8.
  2. Sparagana, S.P. and Roach, E.S. (2000) Curr Opin Neurol 13, 115-9.
  3. Manning, B.D. et al. (2002) Mol Cell 10, 151-62.
  4. Aicher, L.D. et al. (2001) J Biol Chem 276, 21017-21.
  5. Dan, H.C. et al. (2002) J Biol Chem 277, 35364-70.
  6. Goncharova, E.A. et al. (2002) J Biol Chem 277, 30958-67.
  7. Inoki, K. et al. (2002) Nat Cell Biol 4, 648-57.
  8. Inoki, K. et al. (2003) Cell 115, 577-90.
  9. Inoki, K. et al. (2006) Cell 126, 955-68.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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