Cell Signaling Technology

Product Pathways - MAPK Signaling

Anisomycin #2222

apoptosis   c-fos   c-jun   fosB   JunD   p38 MAPK   p70/85 S6 kinase   phorbol esters   phospho junB   SAPK/JNK  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Molecular Characterization






Chemical structure of anisomycin.茴香霉素的化学结构。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, treated with Anisomycin (25 μg/ml) for the indicated times, with or without pretreatment with JNK inhibitor SP600125 (50 μM, 40 min; +), using Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb #8053 (upper) or JunB (C37F9) Rabbit mAb #3753 (lower).Western blot分析293T细胞提取物,先用JNK抑制剂SP600125 (50 μM, 40分钟; +)进行预处理或不预处理,再用茴香霉素(25 μg/ml)根据方案中设计好的时间处理,使用的抗体是:Phospho-JunB (Thr102/Thr104) (D3C6) Rabbit mAb #8053(上图)、JunB (C37F9) Rabbit mAb #3753(下图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, serum-starved overnight, pretreated with the indicated concentrations of SB202190 for 1 hr, and subsequently treated with Anisomycin (25 μg/ml, 30 min; +), using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (upper) or p38 MAPK Antibody #9212 (lower).Western blot分析Jurkat细胞提取物,血清饥饿过夜,先用SB202190按照方案中设计好的浓度预处理1小时,再用茴香霉素(25 μg/ml, 30分钟; +)处理,使用的抗体是:Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511(上图)、p38 MAPK Antibody #9212(下图)。

Directions for Use

Anisomycin is supplied as a lyophilized powder. For a 25 mg/ml stock, reconstitute the 10 mg in 400 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µg/ml for 5-60 minutes. Soluble in DMSO or MeOH.

提供的茴香霉素为冻干粉。对于25 mg/ml浓度贮存液,在10mg冻干粉中加入400 µl DMSO。工作浓度和处理时间根据所需的预期效果来确定,一般是5-50 µg/ml,处理5-60分钟。能溶于DMSO或甲醇。


Anisomycin, an antibiotic produced by Streptomyces griseolus and S. roseochromogenes, was originally described to inhibit protein-protein synthesis at the translational level (1). More recently, it is has been well characterized to strongly activate the stress kinases SAPK/JNK and p38 MAPK, as well as p70/85 S6 kinase in mammalian cells, which results in the rapid induction of immediate-early (IE) genes, such as c-fos, fosB, c-jun, JunB, and JunD (1). Investigators have demonstrated that anisomycin acts as a potent signaling agonist, synergizing with growth factors and phorbol esters to superinduce these IE genes (1,2). Research studies have demonstrated that anisomycin induces apoptosis in many cancer cell lines (3-5).

茴香霉素是一种抗生素,由浅灰链霉菌和玫瑰产色链霉菌产生,能在翻译水平抑制蛋白-蛋白的合成(1)。最近研究发现,它能有力激活应激激酶SAPK/JNK和p38 MAPK以及哺乳动物细胞中的p70/85 S6激酶,导致早期(IE)基因的快速诱导,这些基因包括c-fos, fosB, c-jun, JunB和JunD(1)。研究者已经证明茴香霉素作为一种有效的信号激动剂,能与生长因子和佛波醇酯类一起重复诱导这些IE基因(1,2)。研究还发现茴香霉素能诱导许多癌细胞系的细胞凋亡(3-5)。

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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