Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb #2197

Cds1   chek2   hucds1   rad53  

No. Size Price
2197S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
2197T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
2197 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 62 Rabbit IgG
IP 1:100
IHC-P 1:200
F 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry,

Specificity / Sensitivity

Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb detects endogenous levels of Chk2 only when phosphorylated at Thr68. Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb 能够检测内源性苏氨酸(68位)磷酸化的Chk2蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr68 of human Chk2. 该单克隆抗体是由合成的人源的针对Chk2蛋白苏氨酸(68位)的磷酸化肽段免疫动物生产的。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-Chk2 (Thr68) (C13C1) Rb mAb versus propidium iodide (DNA content). The boxed population indicates phospho-Chk2 (Thr68)-positive cells. 流式细胞术检测未处理的Jurkat细胞,使用的抗体为Phospho-Chk2 (Thr68) (C13C1) Rb mAb,PI染色法检测DNA含量作为对照。框内的细胞群为 phospho-Chk2 (Thr68)阳性细胞。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or UV-treated, using Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb. Western blot方法检测未处理和紫外处理的HeLa细胞提取物,使用的抗体为Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb。



Immunoprecipitation of phospho-chk2 from UV-treated HT29 cells using Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb followed by western blot using the same antibody. 从HT29细胞中免疫沉淀phospho-chk2蛋白,使用的抗体为Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb,随后使用同一抗体进行western blot检测。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb. 免疫组织化学方法检测石蜡包埋人类乳腺癌组织,使用的抗体为Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, control (left) or λ phosphatase-treated (right), using Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb. 免疫组织化学方法检测石蜡包埋人类结肠癌组织,对照组(左图)或λ 磷酸酶粗粒组(右图),使用的抗体为Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HT-29 cell pellets, control (left) or UV-treated (right), using Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb. 免疫组织化学分析石蜡包埋HT-29细胞沉淀,对照组(左图)和紫外处理组(右图),使用的抗体为Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb. 免疫组织化学方法检测石蜡包埋人类肺癌组织,使用的抗体为Phospho-Chk2 (Thr68) (C13C1) Rabbit mAb。


Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain (8). Chk2是哺乳动物中和芽殖酵母Rad53和裂殖酵母Cds1检验点激酶同源的蛋白(1-3)。Chk2的氨基末端结构域包含有一个七丝氨酸或者苏氨酸残基(Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68)组成的系列,每个系列紧跟着谷氨酸(SQ 或TQ模体)。这些地方是已知的ATM/ATR激酶磷酸化的首选作用位点(4,5)。电离辐射(IR)引起DNA损伤之后,UV照射处理或者羟基脲处理,这个区域的68位苏氨酸和其他位点被ATM/ATR磷酸化(5-7)。因此,SQ/TQ簇结构域似乎具有调节功能。第68位苏氨酸的磷酸化是后续激活步骤的先决条件,激活归因于激酶结构域激活回路中Chk2第383位和387位苏氨酸残基的自体磷酸化(8)。

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  2. Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665.
  3. Murakami, H. and Okayama, H. (1995) Nature 374, 817-819.
  4. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.
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  8. Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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