Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

IQGAP1 (D8K4X) XP® Rabbit mAb #20648

No. Size Price
20648S 100 µl ( 10 western blots ) ¥3,750.00 现货查询 购买询价 防伪查询
20648T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价 防伪查询
20648 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 195 Rabbit IgG
IHC-P 1:800
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

IQGAP1 (D8K4X) XP® Rabbit mAb recognizes endogenous levels of total IQGAP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human IQGAP1 protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A549 (left) and Hep G2 (right) cells using IQGAP1 (D8K4X) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using IQGAP1 (D8K4X) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

breast:

Immunohistochemical analysis of paraffin-embedded human infiltrating papillary carcinoma of the breast using IQGAP1 (D8K4X) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded SK-MEL-28 (left) and LNCaP (right) cell pellets using IQGAP1 (D8K4X) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using IQGAP1 (D8K4X) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using IQGAP1 (D8K4X) XP® Rabbit mAb.

Background

IQGAPs are scaffolding proteins involved in mediating cytoskeletal function. They contain multiple protein interaction domains and bind to a growing number of molecules including actin, myosin light chain, calmodulin, E-cadherin, and β-catenin (reviewed in 1). Through their GAP-related domains, they bind the small GTPases Rac1 and cdc42. IQGAPs lack GAP activity, however, and regulate small GTPases by stabilizing their GTP-bound (active) forms (2,3). Research studies have shown that the function and distribution of the IQGAP proteins widely vary. IQGAP1 is ubiquitously expressed and has been found to interact with APC (4) and the CLIP170 complex (5) in response to small GTPases, promoting cell polarization and migration. Additional research studies have suggested that IQGAP1 could play a part in the invasiveness of some cancers (6-8). IQGAP2, which is about 60% identical to IQGAP1, is expressed primarily in liver (3), but lower levels have been detected in the prostate, kidney, thyroid, stomach, and testis (9,10). Research studies have shown that IQGAP2 displays tumor suppressor properties (11). Less is known about the function of IQGAP3, but this protein is present in the lung, brain, small intestine, and testis (9) and is only expressed in proliferating cells (12), suggesting a role in cell growth and division.

  1. Briggs, M.W. and Sacks, D.B. (2003) EMBO Rep 4, 571-4.
  2. Ho, Y.D. et al. (1999) J Biol Chem 274, 464-70.
  3. Brill, S. et al. (1996) Mol Cell Biol 16, 4869-78.
  4. Watanabe, T. et al. (2004) Dev Cell 7, 871-83.
  5. Fukata, M. et al. (2002) Cell 109, 873-85.
  6. Chew, C.S. et al. (2005) Am J Physiol Gastrointest Liver Physiol 288, G376-87.
  7. Jin, S.H. et al. (2008) Int J Cancer 122, 1040-6.
  8. Liu, Z. et al. (2010) Clin Cancer Res 16, 6009-18.
  9. Wang, S. et al. (2007) J Cell Sci 120, 567-77.
  10. Schmidt, V.A. et al. (2003) Blood 101, 3021-8.
  11. Nojima, H. et al. (2008) Nat Cell Biol 10, 971-8.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications