Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) #20194

No. Size Price
20194S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
20194T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
20194 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 50-80 Rabbit IgG
IP 1:50
IF-F 1:200
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) recognizes endogenous levels of tau protein when phosphorylated at Ser404. This antibody detects single phosphorylation at Ser404, dual phosphorylation at Ser400/Ser404 or Thr403/Ser404, and triple phosphorylation at Ser400/Thr403/Ser404. This antibody does not detect peptides with single phosphorylation at Ser400 or Thr403, and dual phosphorylation at Ser400/Thr403.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser400/Thr403/Ser404 of human Tau protein.

IF-IC

IF-IC

Confocal immunofluorescent analysis of mouse primary neurons using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (green). Blue pseudocolor = Hoescht 33342 #4082 (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of Tg2576 mouse brain, untreated (left) or Lambda Protein Phosphatase-treated (right), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (red) and GFAP (GA5) Mouse mAb #3670 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from human cortex (lane 1), neonatal mouse brain, untreated (lane 2) or phosphatase-treated (lane 3), and fetal rat brain (lane 4), using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from MEF cells (lane 1) and mouse brain (lane 2) using Phospho-Tau (Ser404) (D2Z4G) Rabbit mAb (IF preferred) (upper), Tau (Tau46) Mouse mAb #4019 (middle) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, GSK-3, and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease; these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Investigators have shown that tau phosphorylation at Ser404 destabilizes microtubules and that tau is hyperphosphorylated at Ser404 in Alzheimer's disease (4-7).

  1. Johnson, G.V. and Stoothoff, W.H. (2004) J Cell Sci 117, 5721-9.
  2. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
  3. Bramblett, G.T. et al. (1993) Neuron 10, 1089-99.
  4. Shiurba, R.A. et al. (1996) Brain Res 737, 119-32.
  5. Hanger, D.P. et al. (1998) J Neurochem 71, 2465-76.
  6. Evans, D.B. et al. (2000) J Biol Chem 275, 24977-83.
  7. Bertrand, J. et al. (2010) Neuroscience 168, 323-34.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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