Cell Signaling Technology

Product Pathways - Neuroscience

AE1/SLC4A1 (D3X1R) Rabbit mAb (IF Formulated; Rodent Specific) #20112

No. Size Price
20112S 100 µl ( 200 tests ) ¥3,100.00 现货查询 购买询价
20112 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IF-F 1:200 Mouse,Rat, Endogenous Rabbit IgG
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

AE1/SLC4A1 (D3X1R) Rabbit mAb (IF Formulated; Rodent Specific) recognizes endogenous levels of total AE1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala176 of human AE1 protein.

IF-F

IF-F

Confocal immunofluorescent analysis of mouse kidney using AE1/SLC4A1 (D3X1R) Rabbit mAb (IF Formulated; Rodent Specific) (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note AE1 expression in intercalated cells of the collecting duct (left) and erythrocytes (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of mouse whole blood using AE1/SLC4A1 (D3X1R) Rabbit mAb (IF Formulated; Rodent Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Note: AE1 expression in enucleated erythrocytes only.

Background

Anion exchange protein 1 (AE1), also named solute carrier family 4 member 1 (SLC4A1), is an anion transporter that mediates chloride-bicarbonate exchange in the kidney and regulates normal acidification of the urine (1,2). A different isoform of AE1 is a major integral membrane structure protein of erythrocytes, where it plays a critical role in the removal of carbon dioxide from tissues (3). In addition, AE1 is required for normal flexibility and stability of the erythrocyte membrane. Mutations in SLC4A1 can lead to hereditary spherocytosis, ovalocytosis, and distal renal tubular-acidosis (4-7). Other mutations that do not cause disease became novel blood group antigens, which are part of the Diego blood group system (8).

  1. Kopito, R.R. and Lodish, H.F. Nature 316, 234-8.
  2. Kollert-Jöns, A. et al. (1993) Am J Physiol 265, F813-21.
  3. Swenson, E.R. et al. (1993) J Appl Physiol (1985) 74, 838-48.
  4. Jarolim, P. et al. (1991) Proc Natl Acad Sci U S A 88, 11022-6.
  5. Maillet, P. et al. (1995) Br J Haematol 91, 804-10.
  6. Quilty, J.A. et al. (2002) Am J Physiol Renal Physiol 282, F810-20.
  7. Escobar, L. et al. (2013) Nefrologia 33, 289-96.
  8. Figueroa, D. (2013) Immunohematology 29, 73-81.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications