Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

OTUD5 (D8Y2U) Rabbit mAb #20087

DUB-A   DUBA   DUBs   OTUD-5   OTUD5  

No. Size Price
20087S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
20087 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat,Monkey, Endogenous 75 Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Bovine, Dog, Pig,

Specificity / Sensitivity

OTUD5 (D8Y2U) Rabbit mAb recognizes endogenous levels of total OTUD5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human OTUD5 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using OTUD5 (D8Y2U) Rabbit mAb.

IP

IP

Immunoprecipitation of OTUD5 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is OTUD5 (D8Y2U) Rabbit mAb. Western blot analysis was performed using OTUD5 (D8Y2U) Rabbit mAb. Anti-Rabbit HRP-conjugated light chain specific secondary antibody was used for detection.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with siRNA targeting human OTUD5 (+), using OTUD5 (D8Y2U) Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Background

Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes, respectively (1,2). Deubiquitinating enzymes (DUBs) are categorized into five subfamilies based on catalytic domain structure: USP, OTU, MJD, UCH, and JAMM/MPN. OTUD5, also known as DUBA, belongs to the OTU family of DUBs. Phosphorylation of OTUD5 at Ser177 is required to activate its DUB activity (3). Research studies have shown that OTUD5 plays a pivotal role in negatively regulating innate immune responses though its ability to bind and deubiquitinate K63-linked TRAF3, thereby suppressing production of IFN-I by macrophages (4). There is also evidence that OTUD5 participates in the adaptive immune response by negatively regulating the production of pro-inflammatory IL-17 by Th17 cells (5).

  1. Nijman, S.M. et al. (2005) Cell 123, 773-86.
  2. Nalepa, G. et al. (2006) Nat Rev Drug Discov 5, 596-613.
  3. Huang, O.W. et al. (2012) Nat Struct Mol Biol 19, 171-5.
  4. Kayagaki, N. et al. (2007) Science 318, 1628-32.
  5. Rutz, S. et al. (2015) Nature 518, 417-21.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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