Cell Signaling Technology

Product Pathways - Apoptosis

TRAF4 (D1N3A) Rabbit mAb #18527

No. Size Price
18527S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
18527 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 50 Rabbit IgG

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

TRAF4 (D1N3A) Rabbit mAb recognizes endogenous levels of total TRAF4 protein. An unknown background band is detected in some cell lines at 80kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg124 of human TRAF4 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-BR-3 cells, mock transfected (-) or transfected with siRNA against human TRAF4, using TRAF4 (D1N3A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human TRAF4 (hTRAF4-Myc/DDK; +), using TRAF4 (D1N3A) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using TRAF4 (D1N3A) Rabbit mAb.

Background

TRAFs (TNF receptor-associated factors) are a family of multifunctional adaptor proteins that bind to surface receptors and recruit additional proteins to form multiprotein signaling complexes capable of promoting cellular responses (1-3). Members of the TRAF family share a common carboxy-terminal "TRAF domain", which mediates interactions with associated proteins; many also contain amino-terminal Zinc/RING finger motifs. The first TRAFs identified, TRAF1 and TRAF2, were found by virtue of their interactions with the cytoplasmic domain of TNF-receptor 2 (TNFRII) (4). The six known TRAFs (TRAF1-6) act as adaptor proteins for a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress responses.

TRAF4, also referred to as CART1 and MLN62, is a divergent member of the TRAF family with relatively weak binding to TNFR family members (5-7). Interactions have been observed between TRAF4 and the neurotrophin receptor p75-NGFR, lymphotoxin-β receptor, and GITR (8-10). While originally identified in metastatic breast carcinoma, TRAF4 has been shown to contribute to tumor growth and invasion in various cancers including breast, lung and colon (11-13). Expression of Traf4 is induced by the tumor suppressor p53 in response to DNA damage, and can promote apoptosis (14).TRAF4 has also been shown to play a critical role in TGF-β signaling, where it has been found to antagonize the E3 ligase Smurf, resulting in enhanced receptor stabilization driving breast cancer metastasis (15).

  1. Arch, R.H. et al. (1998) Genes Dev. 12, 2821-30.
  2. Chung, J. Y. et al. (2002) J. Cell Sci. 115, 679-88.
  3. Bradley, J.R. and Pober, J.S. (2001) Oncogene 20, 6482-91.
  4. Rothe, M. et al. (1994) Cell 78, 681-92.
  5. Kawamata, S. et al. (1998) J Biol Chem 273, 5808-14.
  6. Régnier, C.H. et al. (1995) J Biol Chem 270, 25715-21.
  7. Bièche, I. et al. (1996) Cancer Res 56, 3886-90.
  8. Yang, K. et al. (2015) Int J Clin Exp Pathol 8, 1419-26.
  9. Camilleri-Broët, S. et al. (2007) Oncogene 26, 142-7.
  10. Li, W. et al. (2013) Cancer Res 73, 6938-50.
  11. Ye, X. et al. (1999) J Biol Chem 274, 30202-8.
  12. Esparza, E.M. and Arch, R.H. (2004) Cell Mol Life Sci 61, 3087-92.
  13. Krajewska, M. et al. (1998) Am J Pathol 152, 1549-61.
  14. Sax, J.K. and El-Deiry, W.S. (2003) J Biol Chem 278, 36435-44.
  15. Zhang, L. et al. (2013) Mol Cell 51, 559-72.

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