Cell Signaling Technology

Product Pathways - Transcription Factors

SIX1 (D5S2S) Rabbit mAb #16960

Sine oculis  

No. Size Price
16960S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
16960 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 36 Rabbit IgG
IP 1:50
IHC-P 1:100
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

SIX1 (D5S2S) Rabbit mAb recognizes endogenous levels of total SIX1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp269 of human SIX1 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded A-204 (left) and HT-29 (right) cell pellets using SIX1 (D5S2S) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle using SIX1 (D5S2S) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse thymus using SIX1 (D5S2S) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle using SIX1 (D5S2S) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SIX1 (D5S2S) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of SIX1 from A-204 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb XP® Isotype Control #3900, and lane 3 is SIX1 (D5S2S) Rabbit mAb. Western blot analysis was performed using SIX1 (D5S2S) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of A-204 cells (left) and Caco-2 cells (right) using SIX1 (D5S2S) Rabbit mAb (green). Actin filaments were labeled with DyLight™ Phalloidin 554 #13054 (red).

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung using SIX1 (D5S2S) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using SIX1 (D5S2S) Rabbit mAb.

Background

Sine oculis homeobox (SIX) proteins belong to a family of evolutionarily conserved transcription factors discovered in Drosophila mutant screens for embryonic eye development genes (1-3). The prototypical family member (sine oculis, so) was named for eyeless embryos carrying mutations in a gene highly conserved among vertebrates, including humans (SIX1) (4). A total of six family members (SIX1-6) have been identified in vertebrates. Each SIX protein contains a homeobox nucleic acid recognition domain (HD) with a DNA-binding helix-turn-helix motif and an adjacent SIX domain, which may be involved in regulating protein-protein interactions (5). In addition to their critical functions during embryonic organogenesis, research studies suggest that SIX proteins play additional roles in postnatal cell cycle regulation, with potentially important implications in tumorigenesis (6,7).

In contrast to the Drosophila ortholog, the vertebrate SIX1 gene product does not play a critical role in embryonic eye development. Vertebrate SIX1 is required for development of mesoderm- and neural crest-derived lineages, and male reproductive tissues (8-10). SIX1 has also been shown to regulate transcription of MyoD in adult muscle progenitor cells during postnatal muscle development (11). A mechanistic role for SIX1 in cell cycle regulation is supported by research studies showing increased SIX1 expression in various cancer subtypes, including breast, ovarian, and hepatocellular carcinoma (6,12,13).

  1. Kumar, J.P. (2009) Cell Mol Life Sci 66, 565-83.
  2. Fischbach, K.F. and Technau, G. (1984) Dev Biol 104, 219-39.
  3. Fischbach, K.F. and Heisenberg, M. (1981) Proc Natl Acad Sci U S A 78, 1105-9.
  4. Boucher, C.A. et al. (1996) Genomics 33, 140-2.
  5. Pignoni, F. et al. (1997) Cell 91, 881-91.
  6. Ford, H.L. et al. (1998) Proc Natl Acad Sci U S A 95, 12608-13.
  7. Coletta, R.D. et al. (2004) Proc Natl Acad Sci U S A 101, 6478-83.
  8. Oliver, G. et al. (1995) Development 121, 4045-55.
  9. Kaiser, R. et al. (2007) Am J Med Genet A 143A, 2185-8.
  10. Fujimoto, Y. et al. (2013) Dev Cell 26, 416-30.
  11. Liu, Y. et al. (2013) PLoS One 8, e67762.
  12. Behbakht, K. et al. (2007) Cancer Res 67, 3036-42.
  13. Ng, K.T. et al. (2006) Br J Cancer 95, 1050-5.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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