Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Ron (C81H9) Rabbit mAb (Biotinylated) #15417

No. Size Price
15417S 100 µl ( 10 western blots ) ¥4,060.00 现货查询 购买询价
15417 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 145, 150 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Ron (C81H9) Rabbit mAb (Biotinylated) recognizes endogenous levels of the β chain of Ron protein. This antibody does not cross-react with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys624 of human Ron protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ron (C81H9) Rabbit mAb #2654.

Western Blotting

Western Blotting

Western blot analysis of extracts from Capan-1 cells using Ron (C81H9) Rabbit mAb (Biotinylated).

Background

Ron is a member of the Met protooncogene family of receptor tyrosine kinases, which also includes Stk, c-Met, and c-Sea. The functional Ron is a heterodimer composed of a 40 kDa α chain and a 150 kDa β chain. Ron is initially synthesized in the cells as a single-chain, pro-Ron precursor that is cleaved into the two active chains. The α chain is completely extracellular, whereas the β chain traverses the cell membrane and contains the intracellular tyrosine kinase and regulatory elements (1,2). Ron mediates multiple signaling cascades that involve cell motility, adhesion, proliferation, and apoptosis. The signaling pathways activated downstream of Ron include the ras/mitogen-activated protein kinase (MAPK), phosphatidyl inositol-3 kinase (PI3K)/Akt, and focal adhesion kinase (FAK) pathways. Ron activation can also significantly increase c-Src activity, a signaling intermediate involved in cell cycle progression, motility, angiogenesis and survival (3,4). The function of Ron has been shown to be important for embryological development as well as implicated in the progression and metastasis of tumors (5).

  1. Ronsin, C. et al. (1993) Oncogene 8, 1195-202.
  2. Gaudino, G. et al. (1994) EMBO J 13, 3524-32.
  3. Wang, M.H. et al. (1996) Oncogene 13, 2167-75.
  4. Danilkovitch-Miagkova, A. (2003) Curr Cancer Drug Targets 3, 31-40.
  5. Leonard, E.J. (1997) Ciba Found Symp 212, 183-91; discussion 192-7.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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