Product Pathways - Lymphocyte Signaling
LAG3 (D2G4O™) XP® Rabbit mAb #15372
|15372S||100 µl ( 10 western blots )||￥3,580.00 现货查询||购买询价|
|15372T||20 µl ( 2 western blots )||￥1,400.00 现货查询||购买询价|
|15372||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),
Specificity / Sensitivity
LAG3 (D2G4O™) XP® recognizes endogenous levels of total LAG3 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human LAG3 protein.
Immunohistochemical analysis of paraffin-embedded HDLM-2 (left) and PC-3 (right) cell pellets on SignalSlide® PD-L1 IHC Controls #13747 using LAG3 (D2G4O™) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human colitis using LAG3 (D2G4O™) XP® Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human tonsil using LAG3 (D2G4O™) XP® Rabbit mAb.
Lymphocyte activation gene 3 (LAG-3, CD223) is an immune checkpoint control protein that negatively regulates T cells and immune responses. A CD4-like member of the Ig superfamily, LAG3 contains an extracellular IgV and three IgC domains, a transmembrane domain, and a short cytoplasmic region (1). LAG3 is primarily expressed by activated CD4+ T cells, CD8+ T cells, Tregs and NK cells, where it's activated by MHC Class II molecules, its only known ligand. While it was initially shown to activate Treg cells (2), LAG3 can also inhibit CD8+ T cells (3,4). LAG3 is often co-expressed with PD-1 on the surface of tumor infiltrating lymphocytes, where the two proteins act independently to contribute to tumor-mediated immune suppression (4,5). Blockade of LAG3 is a promising strategy for neoplastic intervention (6).
- Triebel, F. et al. (1990) J Exp Med 171, 1393-405.
- Huang, C.T. et al. (2004) Immunity 21, 503-13.
- Grosso, J.F. et al. (2007) J Clin Invest 117, 3383-92.
- Woo, S.R. et al. (2012) Cancer Res 72, 917-27.
- Matsuzaki, J. et al. (2010) Proc Natl Acad Sci U S A 107, 7875-80.
- Goldberg, M.V. and Drake, C.G. (2011) Curr Top Microbiol Immunol 344, 269-78.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 12369 Nitrocellulose Sandwiches
- 12630 SignalFire™ Plus ECL Reagent
- 12757 SignalFire™ Elite ECL Reagent
- 13079 Immunohistochemistry Application Solutions Kit (Rabbit)
- 13747 SignalSlide® PD-L1 IHC Controls
- 14166 Hematoxylin
- 14177 SignalStain® Mounting Medium
- 14746 SignalStain® Citrate Unmasking Solution (10X)
- 15019 Animal-Free Blocking Solution (5X)
- 5425 Normal Goat Serum
- 6883 SignalFire™ ECL Reagent
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 8059 SignalStain® DAB Substrate Kit
- 8112 SignalStain® Antibody Diluent
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 9997 Tris Buffered Saline with Tween® 20 (TBST-10X)
- 9998 BSA
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
SignalSlide is a registered trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.
D2G4O is a trademark of Cell Signaling Technology. Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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