Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

LAG3 (D2G4O™) XP® Rabbit mAb #15372

CD223   LAG-3   LAG3  

No. Size Price
15372S 100 µl ( 10 western blots ) ¥3,580.00 现货查询 购买询价
15372T 20 µl ( 2 western blots ) ¥1,400.00 现货查询 购买询价
15372 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 60-80 Rabbit IgG
IHC-P 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

LAG3 (D2G4O™) XP® recognizes endogenous levels of total LAG3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human LAG3 protein.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HDLM-2 (left) and PC-3 (right) cell pellets on SignalSlide® PD-L1 IHC Controls #13747 using LAG3 (D2G4O™) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colitis using LAG3 (D2G4O™) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human tonsil using LAG3 (D2G4O™) XP® Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from HDLM-2, HuT 78, and Jurkat cells using LAG3 (D2G4O™) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Background

Lymphocyte activation gene 3 (LAG-3, CD223) is an immune checkpoint control protein that negatively regulates T cells and immune responses. A CD4-like member of the Ig superfamily, LAG3 contains an extracellular IgV and three IgC domains, a transmembrane domain, and a short cytoplasmic region (1). LAG3 is primarily expressed by activated CD4+ T cells, CD8+ T cells, Tregs and NK cells, where it's activated by MHC Class II molecules, its only known ligand. While it was initially shown to activate Treg cells (2), LAG3 can also inhibit CD8+ T cells (3,4). LAG3 is often co-expressed with PD-1 on the surface of tumor infiltrating lymphocytes, where the two proteins act independently to contribute to tumor-mediated immune suppression (4,5). Blockade of LAG3 is a promising strategy for neoplastic intervention (6).

  1. Triebel, F. et al. (1990) J Exp Med 171, 1393-405.
  2. Huang, C.T. et al. (2004) Immunity 21, 503-13.
  3. Grosso, J.F. et al. (2007) J Clin Invest 117, 3383-92.
  4. Woo, S.R. et al. (2012) Cancer Res 72, 917-27.
  5. Matsuzaki, J. et al. (2010) Proc Natl Acad Sci U S A 107, 7875-80.
  6. Goldberg, M.V. and Drake, C.G. (2011) Curr Top Microbiol Immunol 344, 269-78.

Application References

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Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

XP is a registered trademark of Cell Signaling Technology, Inc.

SignalSlide is a registered trademark of Cell Signaling Technology, Inc.

SignalStain is a trademark of Cell Signaling Technology, Inc.

Tween is a registered trademark of ICI Americas, Inc.

D2G4O is a trademark of Cell Signaling Technology. Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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