Product Pathways - Nuclear Receptor Signaling
|14776S||5 mg||￥636.00 现货查询||购买询价|
|14776||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Western blot analysis of extracts from A549 cells, untreated (-) or treated with Dexamethasone (24 hr) at the indicated concentrations, using β-Arrestin 1 (D8O3J) Rabbit mAb #12697 (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from HeLa cells, serum-starved overnight and untreated (-) or treated with Dexamethasone (1 hr) at the indicated concentrations, using Phospho-Glucocorticoid Receptor (Ser211) Antibody #4161 (upper) and Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 (lower).
Directions for Use
Dexamethasone is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 1.27 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1,000 nM for 1-24 hr.
Dexamethasone is a synthetic glucocorticoid that binds to the human glucocorticoid receptor (GR). Treatment of cells with dexamethasone leads to nuclear translocation and activation of GR. This elicits anti-inflammatory, cellular proliferation, and metabolic responses via GR-mediated transcriptional activation or repression of target genes (1-4). Research studies show that dexamethasone inhibits dendritic cell differentiation and maturation (5). The inhibition of nitric oxide synthase by dexamethasone has also been observed (6).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 12041 Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb
- 12697 β-Arrestin 1 (D8O3J) Rabbit mAb
- 4161 Phospho-Glucocorticoid Receptor (Ser211) Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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