Cell Signaling Technology

Product Pathways - Nuclear Receptor Signaling

Dexamethasone #14776

arrestin   corticosteroid   dexa   glucocorticoid   GR   steroid  

Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype

Species cross-reactivity is determined by western blot.

Applications Key:

Description

Molecular Weight:

392.5 g/mol

Molecular Characterization

C22H29FO5

Purity

>98%

Western Blotting

Western Blotting

Western blot analysis of extracts from A549 cells, untreated (-) or treated with Dexamethasone (24 hr) at the indicated concentrations, using β-Arrestin 1 (D8O3J) Rabbit mAb #12697 (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, serum-starved overnight and untreated (-) or treated with Dexamethasone (1 hr) at the indicated concentrations, using Phospho-Glucocorticoid Receptor (Ser211) Antibody #4161 (upper) and Glucocorticoid Receptor (D6H2L) XP® Rabbit mAb #12041 (lower).

Structure

Structure

Chemical structure of Dexamethasone.

Directions for Use

Dexamethasone is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 1.27 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1,000 nM for 1-24 hr.

Background

Dexamethasone is a synthetic glucocorticoid that binds to the human glucocorticoid receptor (GR). Treatment of cells with dexamethasone leads to nuclear translocation and activation of GR. This elicits anti-inflammatory, cellular proliferation, and metabolic responses via GR-mediated transcriptional activation or repression of target genes (1-4). Research studies show that dexamethasone inhibits dendritic cell differentiation and maturation (5). The inhibition of nitric oxide synthase by dexamethasone has also been observed (6).

Application References

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Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

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