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SignalStain® Citrate Unmasking Solution (10X) #14746
|14746P||25 ml||￥324.00||现货查询 购买询价 防伪查询|
|14746S||125 ml||￥1,180.00||现货查询 购买询价 防伪查询|
|14746||carrier free & custom formulation / quantity||email request|
SignalStain® Citrate Unmasking Solution (10X) is used for antigen unmasking of formalin fixed, paraffin-embedded tissue sections or cell pellets in immunohistochemical assays (IHC-P). Cell Signaling Technology recommends the optimal unmasking reagent for each IHC-P approved antibody. Please consult the primary antibody datasheet to determine if citrate is recommended for your specific product.
This product supplies enough 10X SignalStain® Citrate Unmasking Solution to make 1.25 liters of 1X unmasking solution at pH 6.0.
Directions for Use
Dilute to a 1X unmasking solution using ddH2O just before use. Bring slides to a boil in 1X SignalStain® Citrate Unmasking Solution. Maintain at a sub-boiling temperature for 10 min. Cool slides on bench top for 30 min.
Antigen unmasking protocols utilizing a water bath are not recommended. While antigen unmasking performed with a microwave is preferred, the staining of particular tissues or antigen targets may require the use of a pressure cooker.
Proteins within fixed tissue sections contain chemical cross-links that may prevent antibody access or mask antigen targets in immunohistochemical assays. This limited access may vary among different tissue or antigens. Heat-induced antigen unmasking improves accessibility of antibodies to tissues antigens (1).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 8125 SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
SignalStain is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
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