渲染靶标:SSR
Render Timestamp: 2025-01-21T18:36:11.841Z
Commit: 199712eb9daea12d88cc0e67894a8a09f475f8cb
XML generation date: 2024-09-30 01:54:08.862
Product last modified at: 2025-01-01T09:05:57.217Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DOG1/Anoctamin 1 (D1M9Q) Rabbit mAb #14476

Filter:
  • WB
  • IP
  • IF
Western Blotting Image 1: DOG1/Anoctamin 1 (D1M9Q) Rabbit mAb
Western blot analysis of extracts from various tissues and cell lines using DOG1/Anoctamin 1 (D1M9Q) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

To Purchase # 14476T

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 150
Source/Isotype Rabbit IgG
Application Key:
  • WB-Western Blotting 
  • IP-Immunoprecipitation 
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • H-Human 
  • Related Products

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Immunofluorescence (Immunocytochemistry) 1:1600

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

DOG1/Anoctamin 1 (D1M9Q) Rabbit mAb recognizes endogenous levels of total Anoctamin 1 protein. An unidentified band is detected around 30 kDa in some tissues.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala976 of human anoctamin 1 protein.

Background

Anoctamin 1 is a plasma membrane calcium-activated chloride channel, also known as discovered on gastrointestinal stromal tumors protein 1 (DOG1) or transmembrane protein 16A (TMEM16A) (1). It is essential for chloride secretion from epithelial tissues (2) and has been shown to be a specific marker for cells of Cajal (3), the pacemaker cells that control smooth muscle contraction. Anoctamin 1 knockout mice exhibit an altered gastric smooth muscle rhythmic contraction (4). More recently, research studies have identified anoctamin 1 as a heat sensor in nociceptive neurons (5). Heat above 44ºC triggers anoctamin 1-dependent depolarization, contributing to the mediation of thermal nociception (5).
For Research Use Only. Not For Use In Diagnostic Procedures.
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